Effect of different equilibration times and freezing rates on bull spermatozoa cryotolerance

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F23%3A95191" target="_blank" >RIV/60460709:41210/23:95191 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.11118/actaun.2023.003" target="_blank" >https://doi.org/10.11118/actaun.2023.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.11118/actaun.2023.003" target="_blank" >10.11118/actaun.2023.003</a>

Result language

angličtina

Original language name

Effect of different equilibration times and freezing rates on bull spermatozoa cryotolerance

Original language description

Sperm cryopreservation is a complex process consisting of several steps, the details of which are not yet universally agreed upon. The objective of this study was to evaluate the effects of different equilibration times and freezing rates on post-thaw resilience and functional integrity. Collected semen from five Holstein bulls (30 samples in total) were split into 4 aliquots to produce frozen straws after 2 different equilibration times (2 h and 4 h) and freezing rates (2-phase and 3-phase). After thawing, the sperm characteristics were measured by flow cytometry. A linear model procedure was used to determine the effects of bull, equilibration time, freezing rate and interaction between them, on sperm variables. The evaluated sperm characteristics included spermatozoa with intact plasma and acrosomal membrane (PMAI), PMAI with high mitochondrial membrane potential (HMMP), spermatozoa with plasma membrane damage (PMD), and spermatozoa with acrosome damage (AD) were analysed immediately, 1 h and 2 h after thawing. In conclusion, the extension of the equilibration time from to 4 h in combination with 2-phase freezing rate led to a significant improvement in all measured parameters of frozen-thawed spermatozoa (P < 0.01).

Czech name

—

Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

—

OECD FORD branch

40101 - Agriculture

Project

<a href="/en/project/QK22010270" target="_blank" >QK22010270: Management optimalization of individual reproduction performance in dairy cattle</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis

ISSN

1211-8516

e-ISSN

1211-8516

Volume of the periodical

71

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

35-42

UT code for WoS article

—

EID of the result in the Scopus database

2-s2.0-85150859952