Low-density lipoprotein supplementation improves the quality of Holstein bulls' insemination doses
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F23%3A95184" target="_blank" >RIV/60460709:41210/23:95184 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.17221/223/2022-CJAS" target="_blank" >https://doi.org/10.17221/223/2022-CJAS</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/223/2022-CJAS" target="_blank" >10.17221/223/2022-CJAS</a>
Alternative languages
Result language
angličtina
Original language name
Low-density lipoprotein supplementation improves the quality of Holstein bulls' insemination doses
Original language description
Despite the routine use of cryopreservation in dairy cattle, the subsequent deterioration in the quality of bull insemination doses (IDs) is an incentive to refine cryopreservation protocols, including the composition of the ID. The aim of this study was to examine, using a flow cytometry assay, whether the selected concentrations of low density lipoproteins (LDLs) extracted from hen-egg yolk may improve the post-thaw quality of the ID diluted with a plant-based extender. In total, 30 ejaculates from five sires (six trials per animal) were collected and processed at the Artificial Insemination Centre. The effect of the low density lipoprotein supplementation to the AndroMed (R) diluent at 4 and 8% (v/v) on the cryopreservation efficiency was tested. The effects of the modified extenders were determined on different sperm subpopulations by simultaneous quadruple staining for flow cytometry as the per-centage of sperm with intact plasma membrane and acrosome (PMAI), PMAI sperm showing high mitochondrial membrane potential (HMMP), sperm with plasma membrane damage (PMD), and sperm with acrosome damage (AD). It was observed that the 8% LDL treatment was more effective (P < 0.05) in preserving all the analysed param-eters than AndroMed (R) without any supplementation. The supplementation of the AndroMed (R) extender with 8% LDL resulted in the most optimal values of the PMAI (30.61 +/- 1.13), HMMP (68.81 +/- 1.25), PMD (68.69 +/- 1.14), and AD (38.36 +/- 1.13) compared to the control treatment (24.86 +/- 1.13 for PMAI, 47.79 +/- 1.25 for HMMP, 74.77 +/- 1.14 for PMD, and 42.83 +/- 1.13 for AD. The results of the study also demonstrated a synergistic positive effect of the LDL and soybean lecithin-based diluent on the spermatozoa post-thaw quality and resilience, based on 2 h long incubation. In conclusion, the soybean based semen extender treatment with LDL represents a beneficial tool to mitigate the detrimental effect of cryopreservation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40101 - Agriculture
Result continuities
Project
<a href="/en/project/QK22010270" target="_blank" >QK22010270: Management optimalization of individual reproduction performance in dairy cattle</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Czech Journal of Animal Science
ISSN
1212-1819
e-ISSN
1212-1819
Volume of the periodical
68
Issue of the periodical within the volume
2
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
8
Pages from-to
64-71
UT code for WoS article
000950569800001
EID of the result in the Scopus database
2-s2.0-85151400422