Cryopreservation of European catfish Silurus glanis sperm: Sperm motility, viability, and hatching success of embryos

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12610%2F05%3A00006259" target="_blank" >RIV/60076658:12610/05:00006259 - isvavai.cz</a>

Alternative codes found

RIV/67985904:_____/05:00024865

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Cryopreservation of European catfish Silurus glanis sperm: Sperm motility, viability, and hatching success of embryos

Original language description

The aim of this study was to elaborate cryopreservation methods for ex situ conservation of European catfish. The success of sperm cryopreservation was evaluated by post-thaw sperm motility and velocity, percentage of live spermatozoa and fertility (hatching rates) using frozen/thawed sperm. The best hatching rates of 82-86% were obtained with sperm stored for 5 h before freezing in immobilizing solution and frozen with Me2SO in concentrations of 8, 10, and 12%, or with a mixture of 5% Me2SO and 5% propandiole. These results did not significantly differ from the fresh sperm control sample. The percentage of live spermatozoa in frozen/thawed sperm did not correlate with hatching rate or motility of spermatozoa, but was negatively correlated with velocity of spermatozoa (r = -0.47, P = 0.05). The percentage motility in frozen/thawed sperm ranged from 8 to 62%, when sperm was stored in immobilizing solution 5 h before freezing. The average value in the fresh sperm (control) was 96%. The f

Czech name

Zmrazovani spermii sumce velkeho. Pohiblivost spermii, 6ivotaschopnost a lihnivost embrii.

Czech description

Cílem studie zmrazování spermií sumce velkého bylo nalezení lepší metody ex situ uchování spermií sumce velkého. Výsledek byl hodnocen u rozmrazeného spermatu na podkladě výsledků motility a rychlosti pohybu spermií, procenta živých spermií a líhnivosti.Nejlepší líhnivost na úrovni 82-86 % byla zjištěna u spermatu uchovávaného v imobilizačním roztoku po dobu 5 h a následně zamrazena v kryozkumavkách s objemem 1-1,8 ml zmrazovaného objemu s metanolem v koncentraci 8, 10, 12 % nebo v roztoku obsahující 5% metanolu a 5 % propandiolu. Dosažené výsledky v líhnivosti se statisticky nelišily od líhnivosti při použití čerstvého nezmrazeného spermatu.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GL - Fishery

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cryobiology

ISSN

0011-2240

e-ISSN

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Volume of the periodical

51

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

250-261

UT code for WoS article

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EID of the result in the Scopus database

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