Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897599" target="_blank" >RIV/60076658:12310/18:43897599 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/18:00499985 RIV/60076658:12520/18:43897599

Result on the web

<a href="https://link.springer.com/article/10.1007/s10695-018-0538-5" target="_blank" >https://link.springer.com/article/10.1007/s10695-018-0538-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10695-018-0538-5" target="_blank" >10.1007/s10695-018-0538-5</a>

Result language

angličtina

Original language name

Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation

Original language description

The loss of sperm quality in sterlet (Acipenser ruthenus) due to freeze-thaw process in cryopreservation was investigated in the present study. Two antifreeze proteins (AFPI or AFPIII) were used at different concentrations of 0.1, 1, 10, and 100g/mL. We compared motility, curvilinear velocity, and plasma membrane integrity of fresh, cryopreserved sperm, and sperm cryopreserved in the presence of antifreeze proteins. Fresh sperm (control) had 85 +/- 4% motility and 160 +/- 2m/s curvilinear velocity, respectively. After cryopreservation, the motility of frozen-thawed sperm without addition of antifreeze proteins significantly decreased (44 +/- 9%), compared to the control. The highest motility of frozen-thawed sperm was obtained in cryopreserved sperm with addition of 1g/mL of AFPIII (58 +/- 14%). No significant differences were observed in curvilinear velocity between fresh sperm and cryopreserved sperm with/without addition of AFPI or AFPIII. The flow cytometry analysis revealed that fresh sperm contained 94.5 +/- 6% live cells, while the cryopreserved sperm only contained 26.6 +/- 14% live cells. Supplementation of antifreeze proteins has significantly improved the percentage of live cells in frozen-thawed sperm, except 0.1g/ml of AFPI group. No significant difference in percentage of live cells was detected in the sperm cryopreserved with 10g/mL of AFPI or AFPIII, compared to fresh sperm. Thus, addition of antifreeze proteins to cryopreservation medium could be considered to improve the post-thawed sperm quality of sterlet.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40103 - Fishery

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Fish Physiology and Biochemistry

ISSN

0920-1742

e-ISSN

—

Volume of the periodical

44

Issue of the periodical within the volume

6

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

1527-1533

UT code for WoS article

000453883600009

EID of the result in the Scopus database

2-s2.0-85050196634