Cryopreservation of sterlet, Acipenser ruthenus spermatozoa: Evaluation of quality parameters and fine ultrastructure.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00557228" target="_blank" >RIV/60077344:_____/22:00557228 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12520/22:43904456 RIV/60076658:12310/22:43904456
Result on the web
<a href="https://doi.org/10.3389/fmars.2022.783278" target="_blank" >https://doi.org/10.3389/fmars.2022.783278</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fmars.2022.783278" target="_blank" >10.3389/fmars.2022.783278</a>
Alternative languages
Result language
angličtina
Original language name
Cryopreservation of sterlet, Acipenser ruthenus spermatozoa: Evaluation of quality parameters and fine ultrastructure.
Original language description
The present study aimed to evaluate cryo-injury during the cryopreservation process in sterlet (Acipenser ruthenus) sperm, focusing on ultrastructural characteristics. Post-thaw sperm quality parameters, including total motility rate, curvilinear velocity (VCL), linearity (LIN), plasma membrane integrity, antioxidant status, DNA damage, and fine ultrastructure were examined on fresh and cryopreserved sperm with/without addition of a single optimal dose of AFPI (10 mu g/mL). A lower motility rate, VCL and plasma membrane integrity, and increased DNA damage (p < 0.05) were observed in frozen-thawed spermatozoa with/without AFPI compared to fresh spermatozoa. The morphology and ultrastructure of spermatozoa were affected during the cryopreservation process with/without supplementation of AFPI. Morphological abnormalities were observed in mitochondria (49-54%) and flagellum (55-57%) of cryopreserved spermatozoa with/without AFPI compared to fresh spermatozoa. In conclusion, the morphology and ultrastructure of spermatozoa were slightly changed after cryopreservation of sterlet spermatozoa with/without 10 mu g/mL AFPI.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10617 - Marine biology, freshwater biology, limnology
Result continuities
Project
<a href="/en/project/EF16_025%2F0007370" target="_blank" >EF16_025/0007370: Reproductive and genetic procedures for preserving fish biodiversity and aquaculture</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in marine science
ISSN
2296-7745
e-ISSN
2296-7745
Volume of the periodical
9
Issue of the periodical within the volume
Mar
Country of publishing house
CH - SWITZERLAND
Number of pages
13
Pages from-to
783278
UT code for WoS article
000787204700001
EID of the result in the Scopus database
2-s2.0-85127565237