Cryopreservation of Sterlet, Acipenser ruthenus Spermatozoa: Evaluation of Quality Parameters and Fine Ultrastructure

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F22%3A43904456" target="_blank" >RIV/60076658:12520/22:43904456 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/22:00557228 RIV/60076658:12310/22:43904456
Result on the web
<a href="https://doi.org/10.3389/fmars.2022.783278" target="_blank" >https://doi.org/10.3389/fmars.2022.783278</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fmars.2022.783278" target="_blank" >10.3389/fmars.2022.783278</a>

Result language
angličtina
Original language name
Cryopreservation of Sterlet, Acipenser ruthenus Spermatozoa: Evaluation of Quality Parameters and Fine Ultrastructure
Original language description
The present study aimed to evaluate cryo-injury during the cryopreservation process in sterlet (Acipenser ruthenus) sperm, focusing on ultrastructural characteristics. Post-thaw sperm quality parameters, including total motility rate, curvilinear velocity (VCL), linearity (LIN), plasma membrane integrity, antioxidant status, DNA damage, and fine ultrastructure were examined on fresh and cryopreserved sperm with/without addition of a single optimal dose of AFPI (10 mu g/mL). A lower motility rate, VCL and plasma membrane integrity, and increased DNA damage (p < 0.05) were observed in frozen-thawed spermatozoa with/without AFPI compared to fresh spermatozoa. The morphology and ultrastructure of spermatozoa were affected during the cryopreservation process with/without supplementation of AFPI. Morphological abnormalities were observed in mitochondria (49-54%) and flagellum (55-57%) of cryopreserved spermatozoa with/without AFPI compared to fresh spermatozoa. In conclusion, the morphology and ultrastructure of spermatozoa were slightly changed after cryopreservation of sterlet spermatozoa with/without 10 mu g/mL AFPI.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10613 - Zoology

Project
<a href="/en/project/QK22020144" target="_blank" >QK22020144: Species and technological diversification of productive aquaculture in the Czech Republic with the aim to increase its efficiency and competitiveness</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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