Cryopreservation of sperm in common carp Cyprinus carpio: correlation of sperm motility and hatching of embryos

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A_____%2F02%3A00004204" target="_blank" >RIV/60076658:_____/02:00004204 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12610/02:00003886 RIV/60076658:12610/02:00005782
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Cryopreservation of sperm in common carp Cyprinus carpio: correlation of sperm motility and hatching of embryos
Original language description
In this study, cryopreservation methods were elaborated for ex situ conservation of nine strains of Bohemian common carp. A sperm freezing procedure was developed that resulted in high motility after thawing as well as high values of velocity and percentage of sperm motility, fertilization and hatching yield. Common carp sperm were diluted in Kurokura medium, chilled to 4 oC and dimethylsulfoxide (Me2SO) was added. Cryotubes of sperm with media were then cooled from +4 oC to -9 oC at a rate of 4 oC.min-1, then from -9 oC to -80 oC at a rate of 11 oC.min-1, held for 6 min at -80 oC and finally transferred into liquid N2. The spermatozoa were thawed in a water bath at 35 oC for 110 s and checked for fertilization yield, hatching yield of embryos and larval malformations. Fresh and frozen/thawed sperm were evaluated for the percentage and for the velocity of motile sperm from video frames using image analysis (Linhart et al., 2000). The means with standard deviation (SD) were evaluated fr
Czech name
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Czech description
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Project
<a href="/en/project/GV523%2F97%2FS056" target="_blank" >GV523/97/S056: Popul.diversity of common carp in ČR. Detailed genetic and reproductive characterization of populations - contriution to the phylogenetic understanding ......</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2002
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
XXth Genetics Days (Proceedings of the Intenational Scientifics Conference
ISBN
80-7157-607-7
ISSN
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e-ISSN
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Number of pages
2
Pages from-to
272-273
Publisher name
Mendelova zemědělská a lesnická univerzita
Place of publication
Brno
Event location
Brno
Event date
Sep 12, 2002
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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