Expression and cellular distribution of zona pellucida glycoproteins in canine oocytes before and after in vitro maturation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F14%3A%230001139" target="_blank" >RIV/00027162:_____/14:#0001139 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1017/S0967199414000549" target="_blank" >http://dx.doi.org/10.1017/S0967199414000549</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S0967199414000549" target="_blank" >10.1017/S0967199414000549</a>

Result language

angličtina

Original language name

Expression and cellular distribution of zona pellucida glycoproteins in canine oocytes before and after in vitro maturation

Original language description

This study was aimed at investigating zona pellucida glycoproteins (ZP) ZP2, ZP3 mRNA expression as well as ZP3, ZP4 (ZPB) protein distribution before and after in vitro maturation (IVM) in canine oocytes. The cumulus-oocyte complexes (COCs) were recovered from 27 anoestrous mongrel bitches and matured for 72 h in TCM199 medium. The canine COCs were analysed before and after IVM. Using real-time quantitative polymerase chain reaction (RQ-PCR), both groups of oocytes were analysed for detection of ZP2 and ZP3 mRNA profiles as well as using confocal microscopic analysis for observation of ZP3 and ZP4 protein distribution. In post-IVM canine oocytes an increase in transcript content of ZP2 and ZP3 genes as well as a decrease in ZP3 and ZP4 protein levelswere observed when compared with pre-IVM oocytes. Moreover, the ZP4 protein before IVM was significantly distributed in the peripheral area of cytoplasm, whereas after IVM it was localized rather than in the entire cytoplasm. In contrast,

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GI - Farm animal breeding and farm animal pedigree breeding

OECD FORD branch

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Project

<a href="/en/project/7AMB13PL052" target="_blank" >7AMB13PL052: Cytoplasmic maturation of porcine oocytes during folliculogenesis</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Zygote

ISSN

0967-1994

e-ISSN

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Volume of the periodical

Neuveden

Issue of the periodical within the volume

OCT

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

1-11

UT code for WoS article

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EID of the result in the Scopus database

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