Detection of porcine zona pellucida sperm-binding proteins (ZP2 and ZP3) using antibodies and western blot membrane stripping

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906373" target="_blank" >RIV/62156489:43210/15:43906373 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Detection of porcine zona pellucida sperm-binding proteins (ZP2 and ZP3) using antibodies and western blot membrane stripping

Original language description

The porcine zona pellucida is composed of three glycoproteins called ZP2, ZP3 and ZP4, and the presence of carbohydrate moiety is crucial for sperm-egg recognition. After fertilization, the zona glycoproteins are proteolytically cleaved and occurs so-called zona hardening to prevent polyspermy. But during in vitro fertilization of porcine oocytes matured in vitro this process fails. The ZP2 and ZP3 protein was visualised using selected antibodies ZP2 M-19 and ZP3 N-20. The antibody using ZP2 emerged asnonspecific. However, efficacy of the stripping strategy using mercaptoethanol was demonstrated. Ideal temperature of stripping for zona proteins was determined to be 50oC. Then there is a loss of target protein.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

GG - Zootechnics

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Animal breeding 2015

ISBN

978-80-7509-180-2

ISSN

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e-ISSN

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Number of pages

7

Pages from-to

98-104

Publisher name

Mendelova univerzita v Brně

Place of publication

Brno

Event location

Brno

Event date

Jan 21, 2015

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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