Evaluation of viable Mycobacterium avium subsp paratuberculosis in milk using peptide-mediated separation and Propidium Monoazide qPCR
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F14%3A%230001166" target="_blank" >RIV/00027162:_____/14:#0001166 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/1574-6968.12480" target="_blank" >http://dx.doi.org/10.1111/1574-6968.12480</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/1574-6968.12480" target="_blank" >10.1111/1574-6968.12480</a>
Alternative languages
Result language
angličtina
Original language name
Evaluation of viable Mycobacterium avium subsp paratuberculosis in milk using peptide-mediated separation and Propidium Monoazide qPCR
Original language description
The causative agent of paratuberculosis in ruminants, Mycobacterium avium subsp. paratuberculosis (MAP), although still a matter of debate, has been linked with Crohn's and other human diseases. The availability of rapid methods for assessing the viability of MAP cells in food, in particular milk, could be of great use for risk management in food safety. MAP viability is generally assessed using culture techniques that require prolonged incubation periods for the growth of MAP. To differentiate betweenviable and nonviable MAP cells in milk samples, this study explores the combination of two already described techniques: peptide magnetic bead separation followed by Propidium Monoazide qPCR. Using an Ordinal Multinomial Logistic Regression model to analyze the results obtained after spiking milk samples with mixtures containing different percentages of viable/dead cells, we were able to assess the probability of the viability status of MAP found in milk. This model was applied to contam
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED0006%2F01%2F01" target="_blank" >ED0006/01/01: Center for Advanced Microbiology & Immunology Research in Veterinary Medicine</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FEMS Microbiology Letters
ISSN
0378-1097
e-ISSN
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Volume of the periodical
356
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
127-133
UT code for WoS article
000339383100017
EID of the result in the Scopus database
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