Comparison of filtering methods, filter processing and DNA extraction kits for detection of mycobacteria in water
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F15%3A%230001245" target="_blank" >RIV/00027162:_____/15:#0001245 - isvavai.cz</a>
Alternative codes found
RIV/00027162:_____/15:N0000008
Result on the web
<a href="http://www.aaem.pl/" target="_blank" >http://www.aaem.pl/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5604/12321966.1167707" target="_blank" >10.5604/12321966.1167707</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of filtering methods, filter processing and DNA extraction kits for detection of mycobacteria in water
Original language description
Introduction and objective. Mycobacteria have been isolated from almost all types of natural waters, as well as from man-made water distribution systems. Detection of mycobacteria using PCR has been described in different types of water; however, currently, there is no standardised protocol for the processing of large volumes of water. Material and Methods. In the present study, different filtering methods are tested and optimised for tap or river water filtration up to 10 L, as well as filter processing and DNA isolation using four commercially available kits. Results. The PowerWater DNA isolation kit (MoBio, USA), together with a kit used for soil and other environmental samples (PowerSoil DNA isolation kit, MoBio), had the highest efficiency. Filtration of 10 L of water and elution of the filter in PBS with the addition of 0.05% of Tween 80 is suggested. Conclusions. The described protocol for filter elution is recommended, and the use of the PowerWater DNA isolation kit for the hig
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE
ISSN
1232-1966
e-ISSN
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Volume of the periodical
22
Issue of the periodical within the volume
3
Country of publishing house
PL - POLAND
Number of pages
4
Pages from-to
429-432
UT code for WoS article
000364164500008
EID of the result in the Scopus database
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