Evaluation of DNA extraction methods for PCR-based detection of Listeria monocytogenes from vegetables

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F15%3A%230001256" target="_blank" >RIV/00027162:_____/15:#0001256 - isvavai.cz</a>
Result on the web
<a href="http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1472-765X" target="_blank" >http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1472-765X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/lam.12367" target="_blank" >10.1111/lam.12367</a>

Result language
angličtina
Original language name
Evaluation of DNA extraction methods for PCR-based detection of Listeria monocytogenes from vegetables
Original language description
Epidemiological data indicate that raw vegetables are associated with outbreaks of Listeria monocytogenes. Therefore, there is a demand for the availability of rapid and sensitive methods, such as PCR assays, for the detection and accurate discriminationof L. monocytogenes. However, the efficiency of PCR methods can be negatively affected by inhibitory compounds commonly found in vegetable matrices that may cause false-negative results. Therefore, the sample processing and DNA isolation steps must be carefully evaluated prior to the introduction of such methods into routine practice. In this study, we compared the ability of three column-based and four magnetic bead-based commercial DNA isolation kits to extract DNA of the model micro-organism L. monocytogenes from raw vegetables. The DNA isolation efficiency of all isolation kits was determined using a triplex real-time qPCR assay designed to specifically detect L. monocytogenes. The kit with best performance, the PowerSoil Microbial
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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