Antibody Response of Dogs After Immunisation with Chimeric Vaccine Against Borreliosis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F15%3A%230001317" target="_blank" >RIV/00027162:_____/15:#0001317 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.provac.2015.05.005" target="_blank" >http://dx.doi.org/10.1016/j.provac.2015.05.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.provac.2015.05.005" target="_blank" >10.1016/j.provac.2015.05.005</a>

Result language

angličtina

Original language name

Antibody Response of Dogs After Immunisation with Chimeric Vaccine Against Borreliosis

Original language description

Two chimeric recombinant fusion proteins (ch-rOspC and ch-rOspA) were created. They are composed of the immunodominant domains of OspC and OspA proteins described in the clinically most important strains of Borrelia. The gene constructs for these chimeric proteins were inserted into plasmids pET28 allowing induced gene expressions in a bacterial system. The proteins were expressed in E. coli BL21 strains, purified and used for preparation of the vaccine. One dose of the tested vaccines contained 50 ?g of each relevant protein (ch-rOspC, ch-rOspA, or ch-rOspC + ch-rOspA). PET GEL A (Seppic) or Aluminium hydroxide gel as the immune adjuvants were used. The dogs were vaccinated three times at 21 days intervals subcutaneously or intradermally and unvaccinated controls were also included. The vaccine-elicited serum action antibodies specific to OspA and OspC were determined using in-house ELISA sets. The immunisation induced specific antibody response in the vaccinated animals and OspC and

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EC - Immunology

OECD FORD branch

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Project

<a href="/en/project/TA01011165" target="_blank" >TA01011165: Synthetic multiepitopic vaccine agains boreliosis intended for veterinary applications</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Procedia in Vaccinology

ISSN

1877-282X

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

7

Country of publishing house

US - UNITED STATES

Number of pages

4

Pages from-to

31-34

UT code for WoS article

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EID of the result in the Scopus database

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