Antibody response of dogs after immunisation with chimeric vaccine against borreliosis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F15%3A33157276" target="_blank" >RIV/61989592:15110/15:33157276 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.provac.2015.05.005" target="_blank" >http://dx.doi.org/10.1016/j.provac.2015.05.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.provac.2015.05.005" target="_blank" >10.1016/j.provac.2015.05.005</a>

Result language

angličtina

Original language name

Antibody response of dogs after immunisation with chimeric vaccine against borreliosis

Original language description

Two chimeric recombinant fusion proteins (ch-rOspC and ch-rOspA) were created. They are composed of the immunodominant domains of OspC and OspA proteins described in the clinically most important strains of Bon-elia. The gene constructs for these chimeric proteins were inserted into plasmids pET28 allowing induced gene expressions in a bacterial system. The proteins were expressed in E. coli BL21 strains, purified and used for preparation of the vaccine. One dose of the tested vaccines contained 50 lug of each relevant protein (ch-rOspC, ch-rOspA, or ch-rOspC+chrOspA). PET GEL A (Seppic) or Aluminium hydroxide gel as the immune adjuvants were used. The dogs were vaccinated three times at 21 days intervals subcutaneously or intradermally and unvaccinated controls were also included. The vaccine-elicited serum action antibodies specific to OspA and OspC were determined using in-house ELISA sets. The immunisation induced specific antibody response in the vaccinated animals and OspC and OspA from representative genospecies B. garinii, B. afzelii, and B. burgdorferi sensu stricto were recognized The control dogs were without antibody response. ELISA examination enables determination of specific post-vaccination antibodies against OspA and OspC. Detection of these antibodies and their quantification may be used for evaluation of efficiency of vaccines.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

EC - Immunology

OECD FORD branch

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Project

<a href="/en/project/TA01011165" target="_blank" >TA01011165: Synthetic multiepitopic vaccine agains boreliosis intended for veterinary applications</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Procedia in Vaccinology

ISBN

—

ISSN

1877-282X

e-ISSN

—

Number of pages

4

Pages from-to

31-34

Publisher name

Elsevier BV

Place of publication

Philadephia, USA

Event location

Philadelphia, USA

Event date

Oct 26, 2014

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000361393300005