A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F17%3AN0000015" target="_blank" >RIV/00027162:_____/17:N0000015 - isvavai.cz</a>
Result on the web
<a href="http://journal.frontiersin.org/article/10.3389/fmicb.2017.00108/full" target="_blank" >http://journal.frontiersin.org/article/10.3389/fmicb.2017.00108/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fmicb.2017.00108" target="_blank" >10.3389/fmicb.2017.00108</a>

Result language
angličtina
Original language name
A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything
Original language description
Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety. Although the concept of PCR is relatively simple, there are specific issues in qPCR that developers and users of this technology must bear in mind. These include the use of correct terminology and definitions, understanding of the principle of PCR, difficulties with interpretation and presentation of data, the limitations of qPCR in different areas of microbial diagnostics and parameters important for the description of qPCR performance. It is not our intention in this review to describe every single aspect of qPCR design, optimization, and validation; however, it is our hope that this basic guide will help to orient beginners and users of qPCR in the use of this powerful technique.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming

Project
<a href="/en/project/LO1218" target="_blank" >LO1218: Healthy animal as a source of wholesome food</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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