Fast and Reliable Differentiation of Eight Trichinella Species Using a High Resolution Melting Assay

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F17%3AN0000031" target="_blank" >RIV/00027162:_____/17:N0000031 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/17:00097702
Result on the web
<a href="https://www.nature.com/articles/s41598-017-16329-x" target="_blank" >https://www.nature.com/articles/s41598-017-16329-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-017-16329-x" target="_blank" >10.1038/s41598-017-16329-x</a>

Result language
angličtina
Original language name
Fast and Reliable Differentiation of Eight Trichinella Species Using a High Resolution Melting Assay
Original language description
High resolution melting analysis (HRMA) is a single-tube method, which can be carried out rapidly as an additional step following real-time quantitative PCR (qPCR). The method enables the differentiation of genetic variation (down to single nucleotide polymorphisms) in amplified DNA fragments without sequencing. HRMA has previously been adopted to determine variability in the amplified genes of a number of organisms. However, only one work to date has focused on pathogenic parasites–nematodes from the genus Trichinella. In this study, we employed a qPCR-HRMA assay specifically targeting two sequential gene fragments–cytochrome c oxidase subunit I (COI) and expansion segment V (ESV), in order to differentiate 37 single L1 muscle larvae samples of eight Trichinella species. We show that qPCR-HRMA based on the mitochondrial COI gene allows differentiation between the sequences of PCR products of the same length. This simple, rapid and reliable method can be used to identify at the species level single larvae of eight Trichinella taxa.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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