Adenosine triphosphate analogs can efficiently inhibit the Zika virus RNA-dependent RNA polymerase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F17%3AN0000096" target="_blank" >RIV/00027162:_____/17:N0000096 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/17:00474801 RIV/61388963:_____/17:00474801

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S0166354216306143" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0166354216306143</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.antiviral.2016.11.020" target="_blank" >10.1016/j.antiviral.2016.11.020</a>

Result language

angličtina

Original language name

Adenosine triphosphate analogs can efficiently inhibit the Zika virus RNA-dependent RNA polymerase

Original language description

We describe the expression and purification of an active recombinant Zika virus RNA-dependent RNA polymerase (RdRp). Next, we present the development and optimization of an in vitro assay to measure its activity. We then applied the assay to selected triphosphate analogs and discovered that 2'-C-methylated nucleosides exhibit strong inhibitory activity. Surprisingly, also carbocyclic derivatives with the carbohydrate locked in a North-like conformation as well as a ribonucleotide with a South conformation exhibited strong activity. Our results suggest that the traditional 2'-C-methylated nucleosides pursued in the race for anti-HCV treatment can be superseded by brand new scaffolds in the case of the Zika virus.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10607 - Virology

Project

<a href="/en/project/GA15-09310S" target="_blank" >GA15-09310S: Rational design of phosphatidylinositol 4-kinase IIalpha inhibitors as tools for chemical biology and potential therapeutics</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Antiviral Research

ISSN

0166-3542

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

137

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

3

Pages from-to

131-133

UT code for WoS article

000393005300016

EID of the result in the Scopus database

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