Increased frequency of chromosome congression defects and aneuploidy in mouse oocytes cultured at lower temperature

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F17%3AN0000102" target="_blank" >RIV/00027162:_____/17:N0000102 - isvavai.cz</a>
Result on the web
<a href="http://www.publish.csiro.au/rd/RD15306" target="_blank" >http://www.publish.csiro.au/rd/RD15306</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1071/RD15306" target="_blank" >10.1071/RD15306</a>

Result language
angličtina
Original language name
Increased frequency of chromosome congression defects and aneuploidy in mouse oocytes cultured at lower temperature
Original language description
Optimal culture conditions are essential for successful IVM of mammalian oocytes and for their further development into an embryo. In the present study we used live cell imaging microscopy to assess the effects of suboptimal culture temperature on various aspects of IVM, including duration of meiosis I, dynamics of polar body extrusion, chromosome congression, anaphase-promoting complex/cyclosome (APC/C) activation and aneuploidy. The data showed that even a small deviation from the optimal incubation temperature causes marked changes in the duration and synchronicity of meiosis, APC/C activity and the frequency of chromosome congression and segregation errors. In vitro manipulation and maturation of germ cells is widely used in both human and animal artificial reproduction techniques. Mammalian oocytes are naturally prone to chromosomal segregation errors, which are responsible for severe mental and developmental disorders. The data presented herein demonstrate that exposure of mouse oocytes to suboptimal temperature during manipulation and maturation could further increase the frequency of chromosome segregation defects in these cells.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10604 - Reproductive biology (medical aspects to be 3)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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