Influence of the Gut Microbiota Composition on Campylobacter jejuni Colonization in Chickens

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F17%3AN0000118" target="_blank" >RIV/00027162:_____/17:N0000118 - isvavai.cz</a>

Result on the web

<a href="http://iai.asm.org/content/85/11/e00380-17" target="_blank" >http://iai.asm.org/content/85/11/e00380-17</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/IAI.00380-17" target="_blank" >10.1128/IAI.00380-17</a>

Result language

angličtina

Original language name

Influence of the Gut Microbiota Composition on Campylobacter jejuni Colonization in Chickens

Original language description

The Campylobacter jejuni-host interaction may be affected by the host's gut microbiota through competitive exclusion, metabolites, or modification of the immune response. To understand this interaction, C. jejuni colonization and local immune responses were compared in chickens with different gut microbiota compositions. Birds were treated with an antibiotic cocktail (AT) (experiments 1 and 2) or raised under germfree (GF) conditions (experiment 3). At 18 days posthatch (dph), they were orally inoculated either with 104 CFU of C. jejuni or with diluent. Cecal as well as systemic C. jejuni colonization, T- and B-cell numbers in the gut, and gut-associated tissue were compared between the different groups. Significantly higher numbers of CFU of C. jejuni were detected in the cecal contents of AT and GF birds, with higher colonization rates in spleen, liver, and ileum, than in birds with a conventional gut microbiota (P < 0.05). Significant upregulation of T and B lymphocyte numbers was detected in cecum, cecal tonsils, and bursa of Fabricius of AT or GF birds after C. jejuni inoculation compared to the respective controls (P < 0.05). This difference was less clear in birds with a conventional gut microbiota. Histopathological gut lesions were observed only in C. jejuni-inoculated AT and GF birds but not in microbiota-colonized C. jejuni-inoculated hatchmates. These results demonstrate that the gut microbiota may contribute to the control of C. jejuni colonization and prevent lesion development. Further studies are needed to identify key players of the gut microbiota and the mechanisms behind their protective role.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

<a href="/en/project/ED0006%2F01%2F01" target="_blank" >ED0006/01/01: Center for Advanced Microbiology & Immunology Research in Veterinary Medicine</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Infection and Immunity

ISSN

0019-9567

e-ISSN

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Volume of the periodical

85

Issue of the periodical within the volume

11

Country of publishing house

US - UNITED STATES

Number of pages

15

Pages from-to

e00380-17

UT code for WoS article

000415686700011

EID of the result in the Scopus database

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