Intradermal vaccination combined with oil-based adjuvants: Dendritic cell activation and priming of T-cell response

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000052" target="_blank" >RIV/00027162:_____/18:N0000052 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Intradermal vaccination combined with oil-based adjuvants: Dendritic cell activation and priming of T-cell response
Original language description
To determine the effect of i.d. immunization with oil-based adjuvants on dendritic cell activation and T-cell polarization, two in vivo experiments were performed. Firstly, animals were immunized with model antigen KLH only or KLH combined with different adjuvants: complete or incomplete Freund's adjuvants, Montanide ISA, Emulsigen and Al(OH)3. Blood samples were taken before immunization, three weeks after immunization and two weeks after reimmunization. Levels of IgG1 and IgG2 antibodies in sera were determined by ELISA. Oil-based adjuvants induced high IgG2 primary response suggesting high Th1 cell polarization. The second study was performed to determine the role of dendritic cells on T-cell polarization after i.d. vaccination, local cytokine and chemokine response was tested. Animals were immunized with the same antigen-adjuvant combination and skin biopsies were taken 4 and 24 hours post-immunization for histopathological analyses and quantitative RT-PCR. As expected, oil - based adjuvants induced rapid Th1- priming dendritic cell activation.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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