Self-assembly of extremely uniform polymeric nanoparticles made by microfluidic mixing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000074" target="_blank" >RIV/00027162:_____/18:N0000074 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Self-assembly of extremely uniform polymeric nanoparticles made by microfluidic mixing

Original language description

11th International Conference Nanotechnology for Healthcare: Progress in Recombinant Vaccines, Molecular Adjuvants and Modern Drug Delivery Systems, Telč, 5.-7.6.2018 – lecture. Introduction: Purpose of the study was to prepare highly monodisperse polymeric nanoparticles which could be used as a model to study the effect of particles size to oral mucosa penetration capabilities in/within sublingual vaccination formulations/systems. Methods: Herein model poly lactic-co-glycolic acid (PLGA) nanoparticles were prepared by adapting the so-called nanoprecipitation or solvent displacement process to commercially available microfluidic mixing platform NanoAssemblr (Precision Nanosystems, Canada). Resulting nanoparticles were characterized by Dynamic light scattering (DLS), Nanoparticles Tracking Analysis (NTA) and transmission electron microscopy (TEM) Results: By systematic optimization of main process parameters as total flow rate (TFR), flow rate ratio (FRR), solvent hydrophobicity and polarity, polymer concentration, temperature and stabilizer concentration, the uniform particles were produced (PDI ≤ 0.009) with good reproducibility over wide range of sizes (<50 to >200 nm). Also, the effect of individual process parameters on the shift of ternary phase diagram boundaries was studied in order to understand and clarify particles formation mechanism. Discussion: Obtained results demonstrated that control of mixing kinetic provided by NanoAssembler platform is crucial for an increase of nanoparticles formulation quality in terms of their physicochemical characteristic (size and polydispersity). The quality of prepared nanoparticles was more than sufficient to use them for further study within our new mucoadhesive nanofibre-based sublingual vaccination patch system. Hence it can be assumed that microfluidics will become an indispensable tool for further development on the field of nanomedicine in the future.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

21001 - Nano-materials (production and properties)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů