Preparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imaging

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000193" target="_blank" >RIV/00027162:_____/18:N0000193 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/18:43914712 RIV/00216305:26620/19:PU131634
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Preparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imaging
Original language description
In last decades, a matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) was used for mapping the spatial distribution of different molecules, mainly peptides, proteins, lipids and metabolites, in different types of tissue. Cryosections are the best suited for getting as much information as possible because the preparation of samples for MALDI MSI does not include deparaffinization and antigen-retrieval steps which are needed in case of conventional formalin-fixed and paraffin-embedded (FFPE) tissue sections. To analyse infection markers like interleukins in the tissue sections of porcine lungs affected by Actinobacillus pleuropneumoniae, it was necessary to obtain very well prepared cryosections for MALDI MSI. Therefore, this work was focused on optimization of preparation of frozen porcine lungs and its cryosections with conserved morphological structure.
Czech name
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Czech description
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Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2018: Proceedings of 25th International PhD Students Conference
ISBN
978-80-7509-597-8
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
506-511
Publisher name
Mendel University in Brno
Place of publication
Brno
Event location
Brno
Event date
Nov 7, 2018
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000462205300096

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