Optimalization of cryohistological technique in rat and porcine lungs
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000194" target="_blank" >RIV/00027162:_____/18:N0000194 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/18:43914677
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Optimalization of cryohistological technique in rat and porcine lungs
Original language description
Histological freezing technique is a quick and gentle method of tissue preparation for identifying the microscopic structure. Lung tissue belongs to the less compact structures and its proper histological processing required testing of several available procedures. The optimization of freezing pulmonary tissue leads to more accurate detection of the pathological process in the lungs and better understanding pathogenesis of respiratory diseases of pigs, especially the porcine pneumonia. The aim of this work was to find a way of preserving the morphological structure of the lungs as in a physiological state. We used pulmonary tissue from 4 laboratory rats and 4 domestic pigs and freezing of samples in liquid nitrogen, iso-pentane, n-heptane and the usual process in the freezer. The results show the most appropriate procedure was after isolation of the lungs, filled part of lung over the bronchus by mixture of 1 : 1 Tissue Tek (O.C.T. Compound) with phosphate-buffered saline solution (PBS) and frozen in super-cooled n-heptane placed on dry ice and samples store at -80 °C.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2018: Proceedings of 25th International PhD Students Conference
ISBN
978-80-7509-597-8
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
361-365
Publisher name
Mendel University in Brno
Place of publication
Brno
Event location
Brno
Event date
Nov 7, 2018
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000462205300067