Comparison of cryoprotective methods for histological examination of rat and porcine lung tissue
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F21%3A43920425" target="_blank" >RIV/62156489:43410/21:43920425 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/21:43920425
Result on the web
<a href="https://doi.org/10.2754/avb202190020225" target="_blank" >https://doi.org/10.2754/avb202190020225</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2754/avb202190020225" target="_blank" >10.2754/avb202190020225</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of cryoprotective methods for histological examination of rat and porcine lung tissue
Original language description
Proper histological evaluation of lung tissue and accurate antigen detection by immunodetection techniques require histological tissue processing to preserve tissue reactivity and open alveolar spaces. In this study, we focused on testing and comparing different procedures of tissue cryopreservation. Sucrose or Tissue Tek were used with several methods of freezing samples by supercooled liquids and liquid nitrogen. Changes in tissue caused during the freezing of samples and the effect of cryoprotectants on the tissue were recorded. Rat and porcine pulmonary tissues were used in this experiment. This study aimed to optimize the process of lung cryopreservation with emphasis on enabling proper anatomical evaluation and preserving a high tissue immunoreactivity. The best results were obtained by inflating pulmonary parenchyma with a 1 : 1 mixture of O.C.T. with phosphate buffered saline (PBS) frozen in supercooled n-heptane placed on dry ice. Pulmonary tissue prepared in this way enables to perform proper histological evaluation and to detect target molecules by immunohistochemical analysis.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Veterinaria Brno
ISSN
0001-7213
e-ISSN
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Volume of the periodical
90
Issue of the periodical within the volume
2
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
7
Pages from-to
225-231
UT code for WoS article
000740749900014
EID of the result in the Scopus database
2-s2.0-85111597113