Transcriptional response to organic compounds from diverse gasoline and biogasoline fuel emissions in human lung cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000230" target="_blank" >RIV/00027162:_____/18:N0000230 - isvavai.cz</a>

Alternative codes found

RIV/68378041:_____/18:00493306 RIV/00216208:11310/18:10378423 RIV/68407700:21220/18:00319171 RIV/68407700:21230/18:00319171 RIV/61989100:27200/18:10242332

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0887233318300389?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0887233318300389?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.tiv.2018.02.002" target="_blank" >10.1016/j.tiv.2018.02.002</a>

Result language

angličtina

Original language name

Transcriptional response to organic compounds from diverse gasoline and biogasoline fuel emissions in human lung cells

Original language description

Modern vehicles equipped with Gasoline Direct Injection (GDI) engine have emerged as an important source of particulate emissions potentially harmful to human health. We collected and characterized gasoline exhaust particles (GEPs) produced by neat gasoline fuel (E0) and its blends with 15% ethanol (E15), 25% n-butanol (nBut25) and 25% isobutanol (i-But25). To study the toxic effects of organic compounds extracted from GEPs, we analyzed gene expression profiles in human lung BEAS-2B cells. Despite the lowest GEP mass, n-But25 extract contained the highest concentration of polycyclic aromatic hydrocarbons (PAHs), while i-But25 extract the lowest. Gene expression analysis identified activation of the DNA damage response and other subsequent events (cell cycle arrest, modulation of extracellular matrix, cell adhesion, inhibition of cholesterol biosynthesis) following 4 h exposure to all GEP extracts. The i-But25 extract induced the most distinctive gene expression pattern particularly after 24 h exposure. Whereas E0, E15 and n-But25 extract treatments resulted in persistent stress signaling including DNA damage response, MAPK signaling, oxidative stress, metabolism of PAHs or pro-inflammatory response, i-But25 induced changes related to the metabolism of the cellular nutrients required for cell recovery. Our results indicate that i-But25 extract possessed the weakest genotoxic potency possibly due to the low PAH content.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30108 - Toxicology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Toxicology in Vitro

ISSN

0887-2333

e-ISSN

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Volume of the periodical

48

Issue of the periodical within the volume

April

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

329-341

UT code for WoS article

000428605400033

EID of the result in the Scopus database

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