Transcriptional response to organic compounds from diverse gasoline and biogasoline fuel emissions in human lung cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27200%2F18%3A10242332" target="_blank" >RIV/61989100:27200/18:10242332 - isvavai.cz</a>
Alternative codes found
RIV/68378041:_____/18:00493306 RIV/00216208:11310/18:10378423 RIV/00027162:_____/18:N0000230 RIV/68407700:21220/18:00319171 RIV/68407700:21230/18:00319171
Result on the web
<a href="https://doi.org/10.1016/j.tiv.2018.02.002" target="_blank" >https://doi.org/10.1016/j.tiv.2018.02.002</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.tiv.2018.02.002" target="_blank" >10.1016/j.tiv.2018.02.002</a>
Alternative languages
Result language
angličtina
Original language name
Transcriptional response to organic compounds from diverse gasoline and biogasoline fuel emissions in human lung cells
Original language description
Modern vehicles equipped with Gasoline Direct Injection (GDI) engine have emerged as an important source of particulate emissions potentially harmful to human health. We collected and characterized gasoline exhaust particles (GEPs) produced by neat gasoline fuel (E0) and its blends with 15% ethanol (E15), 25% n-butanol (n-But25) and 25% isobutanol (i-But25). To study the toxic effects of organic compounds extracted from GEPs, we analyzed gene expression profiles in human lung BEAS-2B cells. Despite the lowest GEP mass, n-But25 extract contained the highest concentration of polycyclic aromatic hydrocarbons (PAHs), while i-But25 extract the lowest. Gene expression analysis identified activation of the DNA damage response and other subsequent events (cell cycle arrest, modulation of extracellular matrix, cell adhesion, inhibition of cholesterol biosynthesis) following 4 h exposure to all GEP extracts. The i-But25 extract induced the most distinctive gene expression pattern particularly after 24 h exposure. Whereas E0, E15 and n-But25 extract treatments resulted in persistent stress signaling including DNA damage response, MAPK signaling, oxidative stress, metabolism of PAHs or pro-inflammatory response, i-But25 induced changes related to the metabolism of the cellular nutrients required for cell recovery. Our results indicate that i-But25 extract possessed the weakest genotoxic potency possibly due to the low PAH content. (C) 2018 The Authors
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
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Continuities
O - Projekt operacniho programu
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Toxicology in Vitro
ISSN
0887-2333
e-ISSN
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Volume of the periodical
48
Issue of the periodical within the volume
April
Country of publishing house
GB - UNITED KINGDOM
Number of pages
13
Pages from-to
329-341
UT code for WoS article
000428605400033
EID of the result in the Scopus database
2-s2.0-85041918546