Size-segregated urban aerosol characterization by electron microscopy and dynamic light scattering and influence of sample preparation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000231" target="_blank" >RIV/00027162:_____/18:N0000231 - isvavai.cz</a>
Alternative codes found
RIV/68081731:_____/18:00494587 RIV/00216208:11310/18:10378428
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S135223101830075X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S135223101830075X?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.atmosenv.2018.02.004" target="_blank" >10.1016/j.atmosenv.2018.02.004</a>
Alternative languages
Result language
angličtina
Original language name
Size-segregated urban aerosol characterization by electron microscopy and dynamic light scattering and influence of sample preparation
Original language description
Size-segregated particulate matter (PM) is frequently used in chemical and toxicological studies. Nevertheless, toxicological in vitro studies working with the whole particles often lack a proper evaluation of PM real size distribution and characterization of agglomeration under the experimental conditions. In this study, changes in particle size distributions during the PM sample manipulation and also semiquantitative elemental composition of single particles were evaluated. Coarse (1–10 μm), upper accumulation (0.5–1 μm), lower accumulation (0.17–0.5 μm), and ultrafine (< 0.17 μm) PM fractions were collected by high volume cascade impactor in Prague city center. Particles were examined using electron microscopy and their elemental composition was determined by energy dispersive X-ray spectroscopy. Larger or smaller particles, not corresponding to the impaction cut points, were found in all fractions, as they occur in agglomerates and are impacted according to their aerodynamic diameter. Elemental composition of particles in size-segregated fractions varied significantly. Nssoot occurred in all size fractions. Metallic nanospheres were found in accumulation fractions, but not in ultrafine fraction where ns-soot, carbonaceous particles, and inorganic salts were identified. Dynamic light scattering was used to measure particle size distribution in water and in cell culture media. PM suspension of lower accumulation fraction in water agglomerated after freezing/thawing the sample, and the agglomerates were disrupted by subsequent sonication. Ultrafine fraction did not agglomerate after freezing/thawing the sample. Both lower accumulation and ultrafine fractions were stable in cell culture media with fetal bovine serum, while high agglomeration occurred in media without fetal bovine serum as measured during 24 h.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30304 - Public and environmental health
Result continuities
Project
<a href="/en/project/GBP503%2F12%2FG147" target="_blank" >GBP503/12/G147: Centre for studies on toxicity of nanoparticles</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Atmospheric Environment
ISSN
1352-2310
e-ISSN
1873-2844
Volume of the periodical
178
Issue of the periodical within the volume
April
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
181-190
UT code for WoS article
000428104200020
EID of the result in the Scopus database
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