Detection and molecular characterization of methicillin-resistant Staphylococcus aureus isolated from bulk tank milk of cows, sheep and goats

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000010" target="_blank" >RIV/00027162:_____/19:N0000010 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16270/19:43877938

Result on the web

<a href="https://www.liebertpub.com/doi/10.1089/fpd.2018.2511" target="_blank" >https://www.liebertpub.com/doi/10.1089/fpd.2018.2511</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1089/fpd.2018.2511" target="_blank" >10.1089/fpd.2018.2511</a>

Result language

angličtina

Original language name

Detection and molecular characterization of methicillin-resistant Staphylococcus aureus isolated from bulk tank milk of cows, sheep and goats

Original language description

This study is aimed at detecting and characterizing methicillin-resistant Staphylococcus aureus (MRSA) from bulk tank milk samples of cows, sheep and goats collected from dairy farms in the Czech Republic. Bulk tank milk samples were collected on 49 farms, 14 of which were found to be positive for MRSA. All MRSA isolates were identified by using PCR detection of the species-specific fragment SA442 and mecA gene. The presence of SCCmec, spa type, MLST, enterotoxin encoding genes (ses), virulence genes and φSa3 prophage was assessed and susceptibility to cefoxitin and tetracycline was tested using the disk diffusion method. Three sequence types (ST) containing seven spa types were identified. Twelve isolates belonged to ST398 spa types t011/SCCmec IVa (goat’s milk), t011/SCCmec V (cow’s milk), t034/SCCmec V (cow’s milk), t1456/SCCmec IVa (cow’s milk), t1255/SCCmec V (cow’s milk) and t2346/SCCmec V (cow’s and goat’s milk). Another two isolates belonged to ST5/t3598/SCCmec IVa (cow’s milk) and ST8/t064/SCCmec IVNT (goat’s milk). In six isolates, one or more ses encoding genes (seb, sed, seg, sei, sej) were confirmed. One isolate (ST398/t034/SCCmec V, cow’s milk) harboured the toxic shock toxin syndrome (tst) gene. Another two isolates (ST398/t1456/SCCmec IVa and ST5/t3598/SCCmec IVa, cow's milk) harboured the staphylokinase (sak) gene and φSa3 prophage, and the latter was the only tetracycline susceptible isolate. However, none of the isolates was positive for Panton-Valentine leucocidin (pvl) or exfoliative toxins A & B (eta&b). ST398, was confirmed to be the major clone among all MRSA positive animal species in different herds. This result provides evidence of the wide geographical spread of ST398 across different regions of the Czech Republic and no host preference among the animal species. Therefore, when evaluating the occupational and foodborne risks, MRSA carriage and infection have to be taken into account.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Foodborne Pathogens and Disease

ISSN

1535-3141

e-ISSN

—

Volume of the periodical

16

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

68-73

UT code for WoS article

000451272800001

EID of the result in the Scopus database

2-s2.0-85060379298