Detection and characterization of methicillin-resistant Staphylococcus aureus isolated from milk samples of cows, sheep, and goats in the Czech Republic

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F18%3A43877180" target="_blank" >RIV/62157124:16270/18:43877180 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Detection and characterization of methicillin-resistant Staphylococcus aureus isolated from milk samples of cows, sheep, and goats in the Czech Republic
Original language description
This study aimed to detect and characterize methicillin-resistant S. aureus (MRSA) from bulk tank milk samples of cows, sheep, and goats collected from 49 dairy farms in the Czech Republic. Out of 52 samples, 15 were positive for MRSA. All MRSA isolates were identified using PCR, detection of the species-specific fragment SA442 and mecA gene. SCCmec, spa type, MLST, staphylococcal enterotoxins (SEs), and virulence genes were characterized. Three sequence types (ST) containing seven spa types were identified. Thirteen isolates belonged to ST398 (t011/ IVNT and V, t034/V, t1456/IVa, t1255/V, t2346/IVa and V). Other two isolates belonged to ST5/t3598/V (cow?s milk) and ST8/t064/V (goat?s milk). In six isolates, one or more SEs (seb, sed, seg, sei, sej) have been confirmed. All isolates were negative for the tested virulence genes except one isolate (ST398/t034/V, cow?s milk) which harboured the toxic shock syndrome toxin gene. The major clone, ST398 in different herds, among all MRSA positive animal species were confirmed. This result evidence wide geographical spread of ST398 across the regions in the Czech Republic and no host preference among the animal species.
Czech name
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Czech description
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Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
SBORNÍK PŘÍSPĚVKŮ: XX. Konference mladých vědeckých pracovníků s mezinárodní účastí konaná v roce 100. výročí založení Vysoké školy zvěrolékařské
ISBN
978-80-7305-799-2
ISSN
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e-ISSN
neuvedeno
Number of pages
3
Pages from-to
23-25
Publisher name
Veterinární a farmaceutická univerzita Brno
Place of publication
Brno
Event location
Brno
Event date
May 30, 2018
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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