Directly sequenced genomes of contemporary strains of syphilis reveal recombination-driven diversity in genes encoding predicted surface-exposed antigens
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000059" target="_blank" >RIV/00027162:_____/19:N0000059 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/19:00107834 RIV/65269705:_____/19:00071118
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fmicb.2019.01691/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fmicb.2019.01691/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fmicb.2019.01691" target="_blank" >10.3389/fmicb.2019.01691</a>
Alternative languages
Result language
angličtina
Original language name
Directly sequenced genomes of contemporary strains of syphilis reveal recombination-driven diversity in genes encoding predicted surface-exposed antigens
Original language description
Syphilis, caused by Treponema pallidum subsp. pallidum (TPA), remains an important public health problem with an increasing worldwide prevalence. Despite recent advances in in vitro cultivation, genetic variability of this pathogen during infection is poorly understood. Here we present contemporary and geographically diverse complete treponemal genome sequences isolated directly from patients using a methyl-directed enrichment prior to sequencing. This approach reveals that approximately 50% of the genetic diversity found in TPA is driven by inter- and/or intra-strain recombination events, particularly in strains belonging to one of the defined genetic groups of syphilis treponemes: Nichols-like strains. Recombinant loci were found to encode putative outer-membrane proteins and the recombination variability was almost exclusively found in regions predicted to be at the host-pathogen interface. While genetic recombination in treponemes has been considered an exceptional event, it was observed in this study to an unexpected extent and suggests relevance to the pathogen’s biology. This study reveals the existence of strains with different repertoires of surface-exposed antigens circulating in the current human population, which should be taken into account during syphilis vaccine development.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30303 - Infectious Diseases
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Microbiology
ISSN
1664-302X
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
JUL 2019
Country of publishing house
CH - SWITZERLAND
Number of pages
14
Pages from-to
1961
UT code for WoS article
000477999500001
EID of the result in the Scopus database
2-s2.0-85069481787