Employment of MOL-PCR in bacterial pathogen detection in small ruminants

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000108" target="_blank" >RIV/00027162:_____/19:N0000108 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Employment of MOL-PCR in bacterial pathogen detection in small ruminants
Original language description
xMAP CONNECT, 2019 5th ANNIVERSARY, AMSTERDAM, 5. – 6.11. 2019. In contrast to the detection of pathogens in the human diagnostic, the spectrum of available methods in the veterinary diagnostic is very low. The major consideration for farmers is the price for the test and therefore, multiplexing can decrease expenses. The aim of the study was to prepare a multiplex panel for the detection of Mycobacterium avium subspecies paratuberculosis, Corynebacterium pseudotuberculosis, Mycobacterium bovis and Mycobacterium tuberculosis complex members. These bacterial pathogens play an important role in health of small ruminants with overlap to human health. These selected zoonotic pathogens can be transmitted via colostrum, milk or fecal samples to other animals or can enter to the human food chain. The diagnostic panel intended for small ruminants was based on the MOL-PCR method followed by MagPix analysis. The validation of the multiplex assay was performed on artificially and naturally contaminated milk samples. The validated MOL-PCR assay proved to be suitable for the routine detection and identification of the above mentioned pathogens. This assay will contribute to the quick identification of infected animals and their effective removal from herd.
Czech name
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Czech description
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Project
<a href="/en/project/QK1810212" target="_blank" >QK1810212: Rapid, complex and multiplex methods for simultaneous detection of food-borne pathogens in food of animal and plant origin</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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