Development of detection panel for the simultaneous screening of biothreat agents - B. anthracis, Y. pestis, F. tularensis and Brucella spp. by MOL-PCR method

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000280" target="_blank" >RIV/00027162:_____/19:N0000280 - isvavai.cz</a>

Alternative codes found

RIV/00027162:_____/19:N0000325

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Development of detection panel for the simultaneous screening of biothreat agents - B. anthracis, Y. pestis, F. tularensis and Brucella spp. by MOL-PCR method

Original language description

xMAP CONNECT, 2019 5th ANNIVERSARY, AMSTERDAM, 5. – 6.11. 2019 - poster. Molecular biological methods, such as polymerase chain reaction (PCR), are very important for the rapid detection of pathogenic agents in the human and veterinary field. Early detection is important for the treatment of infectious diseases. Currently, a large number of pathogens need to be identified in a short time in a single reaction. We have developed and validated detection panel based on multiplex method based on oligonucleotide ligation followed by PCR for bacteria that can be used as a biological weapon. The panel detects bacteria, which are classified in category A - most dangerous (B. anthracis, Y. pestis, F. tularensis) and B - less dangerous pathogens (Brucella spp.). For this reason, we used xMAP technology (x = analyte, MAP = Multi Analyte Profiling) using magnetic microspheres to detect nucleic acids (specific genes). Multiplex oligonucleotide ligation (MOL) followed by PCR with fluorescently labelled primer was optimized in this protocol. The final step was the hybridization of PCR products to magnetic microspheres and MagPix analysis. Finally, the designed oligonucleotide probes for detection of selected bacteria were tested and the detection panel was validated to fulfil the requirements for the routine diagnostic method. This includes determination of specificity and sensitivity.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10607 - Virology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů