Genes regulating programmed cell death are significantly upregulated in porcine immature oocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000114" target="_blank" >RIV/00027162:_____/19:N0000114 - isvavai.cz</a>

Alternative codes found

RIV/65269705:_____/19:00071126

Result on the web

<a href="https://content.sciendo.com/view/journals/acb/7/1/article-p1.xml" target="_blank" >https://content.sciendo.com/view/journals/acb/7/1/article-p1.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/acb-2019-0001" target="_blank" >10.2478/acb-2019-0001</a>

Result language

angličtina

Original language name

Genes regulating programmed cell death are significantly upregulated in porcine immature oocytes

Original language description

Correct maturation of the oocyte is crucial for further fertilization and embryogenesis. It comprises of both nuclear and cytoplasmic maturation, during which the proteins, nutrients and mRNAs are assembled. Cumulus cells are connected with the oocyte via gap-junctions, which enable bi-directional transfer of molecules, forming cumulus-oocyte complex (COC). The expression pattern in CCs is thought to resemble the genes expressed in the oocyte. The CCs surrounding the gamete of high developmental competence have an increased expression of apoptotic markers. Therefore, our aim in this study was to determine whether any apoptosis-related genes are upregulated in porcine oocytes before or after IVM. We isolated COCs from 45 pubertal crossbred gilts, performed brilliant cresyl blue (BCB) staining and analyzed the gene expression pattern in oocytes before and after IVM with the use of microarray analysis. The results include 419 differentially expressed transcripts, 25 of which belong to „regulation of apoptosis” and „regulation of cell death” GO BP terms. This set of genes includes BCLAF1, EIF2AK3, KLF10, MIF, MAP3K1, NOTCH2, TXNIP and APP, all of which have been upregulated in immature porcine oocytes. Our results suggest that they play part in porcine oocyte maturation and could be used as potential markers of female gamete’s developmental competence. This knowledge could serve as a basis to improve ART in pigs.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

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OECD FORD branch

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Medical Journal of Cell Biology

ISSN

2544-3577

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

1

Country of publishing house

PL - POLAND

Number of pages

10

Pages from-to

1-10

UT code for WoS article

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EID of the result in the Scopus database

2-s2.0-85069951631