Transcriptomic profile of genes encoding proteins responsible for regulation of cells differentiation and neurogenesis in vivo and in vitro-an oocyte model approach

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F20%3A00074033" target="_blank" >RIV/65269705:_____/20:00074033 - isvavai.cz</a>

Result on the web

<a href="https://content.sciendo.com/view/journals/acb/8/1/article-p1.xml" target="_blank" >https://content.sciendo.com/view/journals/acb/8/1/article-p1.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/acb-2020-0001" target="_blank" >10.2478/acb-2020-0001</a>

Result language

angličtina

Original language name

Transcriptomic profile of genes encoding proteins responsible for regulation of cells differentiation and neurogenesis in vivo and in vitro-an oocyte model approach

Original language description

The growth and development of the oocyte is essential for the ovarian follicle. Cumulus cells (CCs)-a population of granulosa cells-exchange metabolites, proteins and oocyte-derived paracrine factors with the oocyte through gap junctions, to contribute to the competency and health of the oocyte. This bi-directional communication of the cumulus-oocyte complex could be better understood through the micro-analysis of a porcine oocyte gene expression before in vitro maturation (IVM) and after. Additionally, the study of the somatic and gamete cells differentiation capability into neuronal lineage would be promising for future stem cell research as granulosa cells are easily accessible waste material from in vitro fertilization (IVF) procedures. Therefore, in this study, the oocytes of 45 pubertal Landrace gilts were isolated and the protein expression of the COCs were analyzed through micro-analysis techniques. Genes belonging to two ontological groups: neuron differentiation and negative regulation of cell differentiation have been identified which have roles in proliferation, migration and differentiation. Twenty identified porcine oocyte genes (VEGFA, BTG2, MCOLN3, EGR2, TGFBR3, GJA1, FST, CTNNA2, RTN4, MDGA1, KIT, RYK, NOTCH2, RORA, SMAD4, ITGB1, SEMA5A, SMARCA1, WWTR1 and APP) were found to be down-regulated after the transition of IVM compared to in vitro. These results could be applied as gene markers for the proliferation, migration and differentiation occurring in the bi-directional communication between the oocyte and CCs.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Medical Journal of Cell Biology

ISSN

2544-3577

e-ISSN

—

Volume of the periodical

8

Issue of the periodical within the volume

1

Country of publishing house

PL - POLAND

Number of pages

11

Pages from-to

1-11

UT code for WoS article

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EID of the result in the Scopus database

2-s2.0-85085756663