Genetic variety of plasmid mediated colistin resistance in Gram-negative bacteria from raw meat products
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000187" target="_blank" >RIV/00027162:_____/19:N0000187 - isvavai.cz</a>
Result on the web
<a href="https://www.anses.fr/fr/content/protection-de-la-sant%C3%A9-publique-impact-du-s%C3%A9quen%C3%A7age-complet-des-g%C3%A9nomes-des-pathog%C3%A8nes-d" target="_blank" >https://www.anses.fr/fr/content/protection-de-la-sant%C3%A9-publique-impact-du-s%C3%A9quen%C3%A7age-complet-des-g%C3%A9nomes-des-pathog%C3%A8nes-d</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Genetic variety of plasmid mediated colistin resistance in Gram-negative bacteria from raw meat products
Original language description
Foodborne pathogens and whole genome sequencing: impact on public health protection, Paris, France, 26.-28. 3. 2019 – poster. This study was focused on plasmid characteristics of Enterobacteriaceae isolates with mcr-1 gene and one Aeromonas spp. isolate carrying both mcr-3 and mcr-7-like genes obtained from retailed raw meat products. Altogether, 17 colistin resistant isolates carrying mcr genes originated from 12 meat samples (turkey, rabbit and quail meat). The sequencing was performed using Illumina MiSeq and Oxford Nanopore MinION platform. The hybrid assembly of the genomes showed three plasmid types (IncX4, IncHI2 and IncI2) carrying the mcr-1 gene in tested isolates. The most prevalent plasmid type carrying mcr-1 was IncX4 detected in E. coli (n=8) and K. pneumoniae (n=2) isolated from meat products of different origin (Czech Republic, Poland, Germany, Brazil). Four E. coli isolates from turkey meat of Polish origin had localized mcr-1 on plasmid type IncHI2 which varied in their size (between ~153 kb to 284 kb) and carried other resistance genes. The isolates of E. coli and C. braakii from one rabbit meat sample from China carried mcr-1 gene on IncI2 plasmid (~60 kb). The results of this study indicate a possible spread of plasmid mediated colistin resistance via raw meat market between bacteria of different genus. IncX4 plasmids carrying mcr-1 gene were found to be conserved among species of Enterobacteriaceae isolated from raw meat products of different origin. While the most of tested IncX4 and IncI2 plasmids had similar size and carried only mcr-1, the tested IncHI2 plasmids carrying mcr-1 gene were diverse in size and in the content of resistance genes. The presence of mcr-3 and mcr-7-like genes on plasmids in colistin resistant Aeromonas spp. isolate was not confirmed.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/NV18-09-00254" target="_blank" >NV18-09-00254: Sentinel testing survey of plasmid-mediated (transferable) colistin resistance among Enterobacteriaceae in the Czech Republic</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů