Dissemination and comparison of genetic determinants of mcr-mediated colistin resistance in Enterobacteriaceae via retailed raw meat products

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000307" target="_blank" >RIV/00027162:_____/19:N0000307 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/19:00111721 RIV/62157124:16270/19:43878261 RIV/62157124:16810/19:43878261

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fmicb.2019.02824/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fmicb.2019.02824/full</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Dissemination and comparison of genetic determinants of mcr-mediated colistin resistance in Enterobacteriaceae via retailed raw meat products

Original language description

The global food chain may significantly support the dissemination of bacteria resistant to antibiotics around the world. This study was aimed at determining the prevalence and genetic characteristics of Enterobacteriaceae, with mcr-mediated colistin resistance, from retail meat of different origins. Bacteria of Enterobacteriaceae carrying the mcr-1 gene were detected in 21% (18/86) of the examined samples, especially in turkey meat and liver originating from the EU and non-EU countries (19%) and in rabbit meat imported from China (2%). The examined samples of chicken and other poultry meat and liver, pork and beef were negative for the presence of bacteria carrying mcr-1 to mcr-5 genes. A huge number of isolates belonging to E. coli (n=54), K. pneumoniae (n=6) and Citrobacter braakii (n=1) carrying the mcr-1 gene were obtained. Despite the high heterogeneity of the tested isolates, the mcr-1 gene was localized on only three types of plasmids (IncX4, IncHI2, IncI2). The most frequent type of plasmid was IncX4, which carried the mcr-1 gene in 77% of E. coli and K. pneumoniae isolates from turkey meat and liver samples from the Czech Republic, Germany, Poland and Brazil. Our findings indicate a highly probable interspecies transfer of IncX4 and IncI2 plasmids within one sample meat. The co-resistance of plasmid-mediated colistin resistance encoded by the mcr-1 and ESBL genes was detected in 18% of the isolates. Another noteworthy finding was the fosA3 gene coding for fosfomycin resistance in a multi-drug resistant isolate of E. coli from rabbit meat imported from China. The observed high level of Enterobacteriaceae with plasmids carrying mcr-1 gene from retail meat reflects the need for a Europe-wide monitoring of mcr-mediated colistin resistance through the whole food chain.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Microbiology

ISSN

1664-302X

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

DEC 2019

Country of publishing house

CH - SWITZERLAND

Number of pages

10

Pages from-to

2824

UT code for WoS article

000504719200001

EID of the result in the Scopus database

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