Emergence of rare bovine-human reassortant DS-1-like rotavirus A strains with G8P[8] genotype in human patients in the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000191" target="_blank" >RIV/00027162:_____/19:N0000191 - isvavai.cz</a>

Alternative codes found

RIV/00159816:_____/19:00072457 RIV/00216224:14110/19:00108575

Result on the web

<a href="https://www.mdpi.com/1999-4915/11/11/1015" target="_blank" >https://www.mdpi.com/1999-4915/11/11/1015</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/v11111015" target="_blank" >10.3390/v11111015</a>

Result language

angličtina

Original language name

Emergence of rare bovine-human reassortant DS-1-like rotavirus A strains with G8P[8] genotype in human patients in the Czech Republic

Original language description

Group A Rotaviruses (RVA) are the leading cause of acute gastroenteritis in children and a major cause of childhood mortality in low-income countries. RVAs are mostly host-specific, however, interspecies transmission and reassortment between human and animal RVAs contribute significantly to their genetic diversity. We investigated VP7 and VP4 genotypes of RVA isolated from 225 stool specimens collected from Czech patients with gastroenteritis during 2016-2019. The most abundant genotypes were G1P[8] (42.7%), G3P[8] (11.1%), G9P[8] (9.8%), G2P[4] (4.4%), G4P[8] (1.3%), G12P[8] (1.3%) and surprisingly G8P[8] (9.3%). Sequence analysis of G8P[8] revealed the highest nucleotide similarity of all Czech G8 sequences to the G8P[8] rotavirus strains isolated in Vietnam in 2014/2015. The whole genome backbone of the Czech G8 strains was determined with the use of next-generation sequencing as DS-1-like. The phylogenetic analysis of all segments clustered the Czech isolates with RVA strains formerly described in Southeast Asia which had emerged following genetic reassortment between bovine and human RVAs. This is the first time that bovine-human DS 1-like G8P[8] strains are detected at high rate in human patients in Central Europe. Whether the emergence of this unusual genotype reflects establishing of a new RVA strain in the population will require continuous monitoring of rotavirus epidemiology.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10607 - Virology

Project

<a href="/en/project/NV16-29937A" target="_blank" >NV16-29937A: Detailed analysis of human rotavirus infections in the Czech Republic including atypical and emergent strains: development of new diagnostic methods</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Viruses

ISSN

1999-4915

e-ISSN

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Volume of the periodical

11

Issue of the periodical within the volume

11

Country of publishing house

CH - SWITZERLAND

Number of pages

14

Pages from-to

1015

UT code for WoS article

000502292300043

EID of the result in the Scopus database

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