Campylobacter in chicken - critical parameters for international, multicentreevaluation of air sampling and detection methods
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000026" target="_blank" >RIV/00027162:_____/20:N0000026 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0740002020300447" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0740002020300447</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fm.2020.103455" target="_blank" >10.1016/j.fm.2020.103455</a>
Alternative languages
Result language
angličtina
Original language name
Campylobacter in chicken - critical parameters for international, multicentreevaluation of air sampling and detection methods
Original language description
"The present pilot study aimed at evaluating air sampling as a novel method for monitoring Campylobacter in poultry farms. We compared the bacteriological isolation of Campylobacter from boot swabs and air filter samples using ISO 10272–1:2017. A secondary aim was to evaluate the use of molecular methods, i.e. real time PCR, on the same sample set. Samples from 44 flocks from five European countries were collected, and included air samples, in parallel with boot swabs. Campylobacter spp. was isolated from seven of 44 boot swabs from three of five partners using the enrichment method. Two of these positive boot swab samples had corresponding positive air samples. Using enrichment, one positive air sample was negative in the corresponding boot swabs, but Campylobacter spp. was isolated from direct plating of the boot swab sample. One partner isolated Campylobacter spp. from six of 10 boot swabs using direct plating. Overall, 33 air filter samples were screened directly with PCR, returning 14 positive results. In conclusion, there was a lack of correspondence between results from analysis of boot swabs and air filters using ISO 10272–1:2017. In contrast, the combination of air filters and direct real-time PCR might be a way forward. Despite the use of the detailed ISO protocols, there were still sections that could be interpreted differently among laboratories. Air sampling may turn into a multi-purpose and low-cost sampling method that may be integrated into self-monitoring programs."
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10613 - Zoology
Result continuities
Project
—
Continuities
R - Projekt Ramcoveho programu EK
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FOOD MICROBIOLOGY
ISSN
0740-0020
e-ISSN
1095-9998
Volume of the periodical
90
Issue of the periodical within the volume
SEP 2020
Country of publishing house
GB - UNITED KINGDOM
Number of pages
6
Pages from-to
103455
UT code for WoS article
000527627400005
EID of the result in the Scopus database
2-s2.0-85080075447