A multicenter proposal for a fast tool to screen biosecure chicken flocks for the foodborne pathogen campylobacter
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000111" target="_blank" >RIV/00027162:_____/20:N0000111 - isvavai.cz</a>
Result on the web
<a href="https://aem.asm.org/content/86/20/e01051-20" target="_blank" >https://aem.asm.org/content/86/20/e01051-20</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/AEM.01051-20" target="_blank" >10.1128/AEM.01051-20</a>
Alternative languages
Result language
angličtina
Original language name
A multicenter proposal for a fast tool to screen biosecure chicken flocks for the foodborne pathogen campylobacter
Original language description
The present multi-centre study aimed at assessing the performance of air sampling as a novel method for monitoring Campylobacter in biosecured poultry farms. We compared, using a harmonized procedure, the bacteriological isolation protocol (ISO 10272-1:2017), and a single real-time PCR method on air filter samples. Air samples and boot swabs were collected from 62 biosecured flocks from five European countries during the summer of 2019. For air filters, the frequency of PCR-positive findings was statistically significantly higher (n=36; 58%) compared to the cultivation methods (p<0.01; Standardized Residuals). The cultivation protocols (one with Bolton enrichment and one with Preston enrichment) were comparable to each other but returned fewer positive samples (0%-8%). The association between type of sample and frequency of PCR-positive findings was confirmed (p<0.01; Fisher´s exact test), although no culture-positive air filters were detected using direct plating. For the boot swabs, the highest number of positive samples were detected after enrichment in Preston broth (n=23; 37%), followed by direct plating after homogenization in Preston (n=21; 34%) or Bolton (n=20; 32%). In conclusion, air sampling combined with real time PCR is proposed as a multi-purpose, low-cost and convenient screening method that can be up to 4 times faster and 4 times more sensitive than the current boot-swab testing scheme used for screening biosecured broiler production. It is important to note that the Norwegian flocks, a country known to be free of Campylobacter in biosecured chicken production, still appeared Campylobacter-free in the new sensitive method.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
<a href="/en/project/ED2.1.00%2F19.0385" target="_blank" >ED2.1.00/19.0385: Development of research capacities for AdmireVet</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Applied and environmental microbiology
ISSN
0099-2240
e-ISSN
1098-5336
Volume of the periodical
86
Issue of the periodical within the volume
20
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
"e01051-20"
UT code for WoS article
000582928300006
EID of the result in the Scopus database
2-s2.0-85092682418