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A multicenter proposal for a fast tool to screen biosecure chicken flocks for the foodborne pathogen campylobacter

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000111" target="_blank" >RIV/00027162:_____/20:N0000111 - isvavai.cz</a>

  • Result on the web

    <a href="https://aem.asm.org/content/86/20/e01051-20" target="_blank" >https://aem.asm.org/content/86/20/e01051-20</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1128/AEM.01051-20" target="_blank" >10.1128/AEM.01051-20</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    A multicenter proposal for a fast tool to screen biosecure chicken flocks for the foodborne pathogen campylobacter

  • Original language description

    The present multi-centre study aimed at assessing the performance of air sampling as a novel method for monitoring Campylobacter in biosecured poultry farms. We compared, using a harmonized procedure, the bacteriological isolation protocol (ISO 10272-1:2017), and a single real-time PCR method on air filter samples. Air samples and boot swabs were collected from 62 biosecured flocks from five European countries during the summer of 2019. For air filters, the frequency of PCR-positive findings was statistically significantly higher (n=36; 58%) compared to the cultivation methods (p<0.01; Standardized Residuals). The cultivation protocols (one with Bolton enrichment and one with Preston enrichment) were comparable to each other but returned fewer positive samples (0%-8%). The association between type of sample and frequency of PCR-positive findings was confirmed (p<0.01; Fisher´s exact test), although no culture-positive air filters were detected using direct plating. For the boot swabs, the highest number of positive samples were detected after enrichment in Preston broth (n=23; 37%), followed by direct plating after homogenization in Preston (n=21; 34%) or Bolton (n=20; 32%). In conclusion, air sampling combined with real time PCR is proposed as a multi-purpose, low-cost and convenient screening method that can be up to 4 times faster and 4 times more sensitive than the current boot-swab testing scheme used for screening biosecured broiler production. It is important to note that the Norwegian flocks, a country known to be free of Campylobacter in biosecured chicken production, still appeared Campylobacter-free in the new sensitive method.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    40301 - Veterinary science

Result continuities

  • Project

    <a href="/en/project/ED2.1.00%2F19.0385" target="_blank" >ED2.1.00/19.0385: Development of research capacities for AdmireVet</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Applied and environmental microbiology

  • ISSN

    0099-2240

  • e-ISSN

    1098-5336

  • Volume of the periodical

    86

  • Issue of the periodical within the volume

    20

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    10

  • Pages from-to

    "e01051-20"

  • UT code for WoS article

    000582928300006

  • EID of the result in the Scopus database

    2-s2.0-85092682418