Minimal Concentrations of Deoxynivalenol Reduce Cytokine Production in Individual Lymphocyte Populations in Pigs
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000035" target="_blank" >RIV/00027162:_____/20:N0000035 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2072-6651/12/3/190" target="_blank" >https://www.mdpi.com/2072-6651/12/3/190</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/toxins12030190" target="_blank" >10.3390/toxins12030190</a>
Alternative languages
Result language
angličtina
Original language name
Minimal Concentrations of Deoxynivalenol Reduce Cytokine Production in Individual Lymphocyte Populations in Pigs
Original language description
Deoxynivalenol (DON) is a mycotoxin frequently found in cereals, and pigs are one of the most sensitive farm species to DON. The aim of this study was to determine the effects of DON in very low doses on peripheral blood mononuclear cells (PBMC) and on particular lymphocyte subpopulations. The cells were exposed to 1, 10 and 100 ng/mL of DON and lymphocyte viability, proliferation, and cytokine (Interleukin (IL)-1 beta, IL-2, IL-8, IL-17, Interferon (IFN) gamma and tumor necrosis factor (TNF) alpha production were studied. Cells exposed to DON for 5 days in concentrations of 1 and 10 ng/mL showed higher viability compared to control cells. After 18 h of DON (100 ng/mL) exposure, a significantly lower proliferation after mitogen stimulation was observed. In contrast, an increase of spontaneous proliferation induced by DON (100 ng/mL) was detected. After DON exposure, the expression of cytokine genes decreased, with the exception of IL-1 beta and IL-8, which increased after 18 h exposure to 100 ng/mL of DON. Among lymphocyte subpopulations, helper T-cells and gamma delta T-cells exhibiting lower production of IL-17, IFN gamma and TNF alpha were most affected by DON exposure (10 ng/mL). These findings show that subclinical doses of DON lead to changes in immune response.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
TOXINS
ISSN
2072-6651
e-ISSN
2072-6651
Volume of the periodical
12
Issue of the periodical within the volume
3
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
"190"
UT code for WoS article
000525009500018
EID of the result in the Scopus database
2-s2.0-85082148160