Profiling of metabolomic changes in plasma and urine of pigs caused by illegal administration of testosterone esters

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000037" target="_blank" >RIV/00027162:_____/20:N0000037 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16270/20:43878743

Result on the web

<a href="https://www.mdpi.com/2218-1989/10/8/307/htm" target="_blank" >https://www.mdpi.com/2218-1989/10/8/307/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/metabo10080307" target="_blank" >10.3390/metabo10080307</a>

Result language

angličtina

Original language name

Profiling of metabolomic changes in plasma and urine of pigs caused by illegal administration of testosterone esters

Original language description

"The use of anabolic steroid hormones as growth promoters in feed for farm animals has been banned in the European Union since 1988 on the basis of Council Directive 96/22/EC. However, there is still ongoing monitoring and reporting of positive findings of these banned substances in EU countries. The aim of this work was to investigate the efficacy and discriminatory ability of metabolic fingerprinting after the administration of 17β-testosterone esters to pigs. Plasma and urine samples were chromatographically separated on a Hypersil Gold C18 column. High resolution mass spectrometry metabolomic fingerprints were analysed on a hybrid mass spectrometer Q-Exactive. Three independent multivariate statistical methods, namely principal component analysis, clustre analysis, and orthogonal partial least squares discriminant analysis showed significant differences between the treated and control groups of pigs even 14 days after the administration of the hormonal drug. Plasma samples were also analysed by a conventional quantitative analysis using liquid chromatography with tandem mass spectrometry and a pharmacokinetic curve was constructed based on the results. In this case, no testosterone residue was detected 14 days after the administration. The results clearly showed that a metabolomics approach can be a useful and effective tool for the detection and monitoring of banned anabolic steroids used illegally in pig fattening."

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Metabolites

ISSN

2218-1989

e-ISSN

—

Volume of the periodical

10

Issue of the periodical within the volume

8

Country of publishing house

CH - SWITZERLAND

Number of pages

24

Pages from-to

"307"

UT code for WoS article

000564062800001

EID of the result in the Scopus database

2-s2.0-85088995642