Proinflammatory effect of carbon-based nanomaterials: in vitro study on stimulation of inflammasome NLRP3 via destabilisation of lysosomes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000090" target="_blank" >RIV/00027162:_____/20:N0000090 - isvavai.cz</a>
Alternative codes found
RIV/68378271:_____/20:00524868 RIV/61388955:_____/20:00524868 RIV/00179906:_____/20:10411311 RIV/00216208:11150/20:10411311 and 2 more
Result on the web
<a href="https://www.mdpi.com/2079-4991/10/3/418" target="_blank" >https://www.mdpi.com/2079-4991/10/3/418</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/nano10030418" target="_blank" >10.3390/nano10030418</a>
Alternative languages
Result language
angličtina
Original language name
Proinflammatory effect of carbon-based nanomaterials: in vitro study on stimulation of inflammasome NLRP3 via destabilisation of lysosomes
Original language description
Carbon-based nanomaterials (C-BNM) have recently attracted an increased attention as the materials with potential applications in industry and medicine. Bioresistance and proinflammatory potential of C-BNM is the main obstacle for their medicinal application which was documented in vivo and in vitro. However, there are still limited data especially on graphene derivatives such as graphene platelets (GP). In this work, we compared multi-walled carbon nanotubes (MWCNT) and two different types of pristine GP in their potential to activate inflammasome NLRP3 (The nod-like receptor family pyrin domain containing 3) in vitro. Our study is focused on exposure of THP-1/THP1-null cells and peripheral blood monocytes to C-BNM as representative models of canonical and alternative pathways, respectively. Although all nanomaterials were extensively accumulated in the cytoplasm, increasing doses of all C-BNM did not lead to cell death. We observed direct activation of NLRP3 via destabilization of lysosomes and release of cathepsin B into cytoplasm only in the case of MWCNTs. Direct activation of NLRP3 by both GP was statistically insignificant but could be induced by synergic action with muramyl dipeptide (MDP), as a representative molecule of the family of pathogen-associated molecular patterns (PAMPs). This study demonstrates a possible proinflammatory potential of GP and MWCNT acting through NLRP3 activation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
21001 - Nano-materials (production and properties)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
NANOMATERIALS
ISSN
2079-4991
e-ISSN
2079-4991
Volume of the periodical
10
Issue of the periodical within the volume
3
Country of publishing house
CH - SWITZERLAND
Number of pages
18
Pages from-to
"418"
UT code for WoS article
000526090400018
EID of the result in the Scopus database
2-s2.0-85080938878