Activation of NLRP3 inflammasome as a key indicator of carbon nanotubes proinflammatory potential

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000225" target="_blank" >RIV/00027162:_____/19:N0000225 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Activation of NLRP3 inflammasome as a key indicator of carbon nanotubes proinflammatory potential

Original language description

The aim of our study was to evaluate pro-inflammatory potential of multi-walled carbon nanotubes (MWCNTs). Despite the fact, that some of these nanotubes are not acutely cytotoxic in vitro, mechanical disruption of some organelles like lysosomes can trigger NLRP3 assembly leading to production of proinflammatory cytokines IL-1β and IL-18. We regard NLRP3 activation as an essential step in induction of chronic inflammation in vivo. Pristine PMWCNTs and functionalized NDMWCNTs and COOHMWCNTs were tested in our study. Detailed observation of shape and length of MWCNTs was assessed using electron microscopy. Human monocyte-derived cell lines THP-1, THP1-null and THP1-defNLRP3 were used as in vitro model. Cell viability was evaluated via LDH assay. Localization of MWCNTs was detected by electron microscopy. Activation of NLRP3 and release of IL-1β was measured by cell-based assay using HEKBlue ™ IL-1β cells. ELISA kits were used for detection of other cytokines in supernatants. After 24h, MWCNTs were located as free particles in the whole cytoplasm. Rarely, some individual tubes were observed to reach the nucleus membrane. Interestingly, while all types of MWCNTs activated NLRP3, only COOHMWCNTs caused release of proinflammatory cytokines IL-6 and TNF-α. Increased LDH levels showed higher cytotoxicity in the case of functionalized MWCNTs only.

Czech name

—

Czech description

—

Type

D - Article in proceedings

CEP classification

—

OECD FORD branch

21001 - Nano-materials (production and properties)

Project

<a href="/en/project/EF15_003%2F0000495" target="_blank" >EF15_003/0000495: FIT (Pharmacology, Immunotherapy, nanoToxicology)</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

EUROPEAN JOURNAL OF IMMUNOLOGY

ISBN

—

ISSN

0014-2980

e-ISSN

1521-4141

Number of pages

1

Pages from-to

601-601

Publisher name

—

Place of publication

—

Event location

Beijing, PEOPLES R CHINA

Event date

Oct 19, 2019

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000490026901573