Efficiency of DNA isolation methods based on silica columns and magnetic separation tested for the detection of Mycobacterium avium subsp. paratuberculosis in milk and faeces.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000139" target="_blank" >RIV/00027162:_____/20:N0000139 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/20:00117380 RIV/62157124:16270/20:43878495

Result on the web

<a href="https://www.mdpi.com/1996-1944/13/22/5112" target="_blank" >https://www.mdpi.com/1996-1944/13/22/5112</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ma13225112" target="_blank" >10.3390/ma13225112</a>

Result language

angličtina

Original language name

Efficiency of DNA isolation methods based on silica columns and magnetic separation tested for the detection of Mycobacterium avium subsp. paratuberculosis in milk and faeces.

Original language description

Timely and reliable detection of animals shedding Mycobacterium avium subsp. paratuberculosis (MAP) should help to effectively identify infected animals and limit infection transmission at early stages to ensure effective control of paratuberculosis. The aim of the study was to compare DNA extraction methods and evaluate isolation efficiency using milk and faecal samples artificially contaminated by MAP with a focus on modern instrumental automatic DNA isolation procedures based on magnetic separation. In parallel, an automatic and manual version of magnetic separation and two methods of faecal samples preparation were compared. Commercially available DNA isolation kits were evaluated, and selected kits were used in a trial of automatic magnetic beads-based isolation and compared with the manual version of each kit. Detection of the single copy element F57 was performed by qPCR to quantify MAP and determine the isolation efficiency. The evaluated kits showed significant differences in DNA isolation efficiencies. The best results were observed with the silica column Blood and tissue kit for milk and Zymo Research for faeces. The highest isolation efficiency for magnetic separation was achieved with MagMAX for both matrices. The magnetic separation and silica column isolation methods used in this study represent frequently used methods in mycobacterial diagnostics.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40301 - Veterinary science

Project

<a href="/en/project/QK1910082" target="_blank" >QK1910082: Addressing the problem of the occurrence of bacterial, protozoan and viral zoonotic agents in small ruminant breeds</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Materials

ISSN

1996-1944

e-ISSN

—

Volume of the periodical

13

Issue of the periodical within the volume

22

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

"5112"

UT code for WoS article

000594883700001

EID of the result in the Scopus database

2-s2.0-85096126884