Molecular Methods for the Detection of Toxoplasma gondii Oocysts in Fresh Produce: An Extensive Review
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000006" target="_blank" >RIV/00027162:_____/21:N0000006 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2076-2607/9/1/167" target="_blank" >https://www.mdpi.com/2076-2607/9/1/167</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/microorganisms9010167" target="_blank" >10.3390/microorganisms9010167</a>
Alternative languages
Result language
angličtina
Original language name
Molecular Methods for the Detection of Toxoplasma gondii Oocysts in Fresh Produce: An Extensive Review
Original language description
Toxoplasma gondii is an important zoonotic foodborne pathogen worldwide and infection in humans has been associated with the consumption of unwashed raw fresh produce. The estimation of relative importance of fresh produce as an infection source is however hampered by the lack of a standardised detection method Aim To support method development and standardisation, we aimed to summarize the key aspects of published methods for the detection of T. gondii oocyst contamination in fresh produce. Methods We performed an extensive literature review and a multi-attribute assessment of published T. gondii oocyst detection methods, irrespective of the contamination matrix, by focusing on three steps: oocyst recovery, DNA extraction, and DNA amplification. The literature review was further supplemented with input from a questionnaire study administered online to 24 expert laboratories worldwide. Results Seventy-seven published studies were included, with 14 focusing on fresh produce. Procedures for oocyst recovery from fresh produce mostly involved sample washing and pelleting of the washing eluate by centrifugation, although washing procedures and buffers varied. DNA extraction procedures including mechanical or thermal shocks were identified as necessary step to break the robust oocyst wall. The most suitable DNA detection protocols rely on qPCR, mostly targeting the B1 gene or the 5 29 bp repetitive element. When reported, validation data for the different detection methods were not comparable and none of the methods was supported by an interlaboratory comparative study. Conclusions The key step to enable detection is oocyst recovery, followed by an efficient DNA extraction and sensitive and specific amplification of the parasite’s DNA. The results of this review will pave the way for an ongoing development of a widely applicable standard operating procedure.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30310 - Parasitology
Result continuities
Project
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Continuities
R - Projekt Ramcoveho programu EK
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microorganisms
ISSN
2076-2607
e-ISSN
2076-2607
Volume of the periodical
9
Issue of the periodical within the volume
1
Country of publishing house
CH - SWITZERLAND
Number of pages
19
Pages from-to
"167"
UT code for WoS article
000610595900001
EID of the result in the Scopus database
2-s2.0-85099562587