Development, inter-laboratory SOP evaluation and application of a molecular method for detection of Toxoplasma gondii oocysts in ready-to-eat leafy-green salads in a multicentre study in Europe
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000117" target="_blank" >RIV/00027162:_____/22:N0000117 - isvavai.cz</a>
Result on the web
<a href="http://www.apicowplexa.net/index.php/bern-2021/" target="_blank" >http://www.apicowplexa.net/index.php/bern-2021/</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Development, inter-laboratory SOP evaluation and application of a molecular method for detection of Toxoplasma gondii oocysts in ready-to-eat leafy-green salads in a multicentre study in Europe
Original language description
Toxoplasma gondii is a zoonotic pathogen with up to 60% of acquired infections associated with foodborne transmission. Consumption of raw fresh produce contaminated with T. gondii oocysts is among the infection routes. However, the relative importance of fresh produce for human infection is underestimated as standardized detection methods are missing. The aim of this study was to develop a standard operating procedure (SOP) for molecular detection of T. gondii oocysts in leafy green salads, implement it in several laboratories, validate it by interlaboratory analysis, and apply it in a multicentre study on ready-to-eat (RTE) salad at European level ensuring delivery of robust results.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
30304 - Public and environmental health
Result continuities
Project
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Continuities
R - Projekt Ramcoveho programu EK
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů