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Development, inter-laboratory SOP evaluation and application of a molecular method for detection of Toxoplasma gondii oocysts in ready-to-eat leafy-green salads in a multicentre study in Europe

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000117" target="_blank" >RIV/00027162:_____/22:N0000117 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.apicowplexa.net/index.php/bern-2021/" target="_blank" >http://www.apicowplexa.net/index.php/bern-2021/</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Development, inter-laboratory SOP evaluation and application of a molecular method for detection of Toxoplasma gondii oocysts in ready-to-eat leafy-green salads in a multicentre study in Europe

  • Original language description

    Toxoplasma gondii is a zoonotic pathogen with up to 60% of acquired infections associated with foodborne transmission. Consumption of raw fresh produce contaminated with T. gondii oocysts is among the infection routes. However, the relative importance of fresh produce for human infection is underestimated as standardized detection methods are missing. The aim of this study was to develop a standard operating procedure (SOP) for molecular detection of T. gondii oocysts in leafy green salads, implement it in several laboratories, validate it by interlaboratory analysis, and apply it in a multicentre study on ready-to-eat (RTE) salad at European level ensuring delivery of robust results.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

  • OECD FORD branch

    30304 - Public and environmental health

Result continuities

  • Project

  • Continuities

    R - Projekt Ramcoveho programu EK

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů