Production of sexed bovine embryos in vitro can be improved by selection of sperm treatment and co-culture system
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000082" target="_blank" >RIV/00027162:_____/21:N0000082 - isvavai.cz</a>
Alternative codes found
RIV/67985904:_____/21:00543643
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1111/rda.13926" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/rda.13926</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/rda.13926" target="_blank" >10.1111/rda.13926</a>
Alternative languages
Result language
angličtina
Original language name
Production of sexed bovine embryos in vitro can be improved by selection of sperm treatment and co-culture system
Original language description
The study investigated the effects of sperm sorting, capacitation treatment and co-cultivation on sexed bovine in vitro embryo production. The effect of treatment and co-culture on production of embryos of the preferred sex from unsorted sperm was also studied. Sperm from five breeding bulls was used for fertilization of mature oocytes as follows: Experiment 1, sorted and unsorted sperm (bulls A-E) treated only with heparin in standard co-cultures; Experiment 2, sorted sperm (bulls A-E) treated with heparin-PHE (penicillamine, hypotaurine, and epinephrine) or heparin-caffeine in drop co-cultures; and Experiment 3, unsorted sperm (bull E) treated with either heparin-PHE or heparin-caffeine in both standard and drop co-cultures. In all bulls, treatment with heparin resulted in significantly (p < .05) reduced cleavage and blastocyst rates from sorted sperm, as compared with those from unsorted sperm. In bulls A, B, D and E, treatment of sorted sperm with heparin-PHE in drops significantly increased the blastocyst rate (p < .05). In unsorted sperm of bull E, heparin-PHE treatment in drops resulted in the XX/XY sex ratio inverse to that obtained by heparin-caffeine treatment in standard co-cultures (32.3%/67.7% and 66.7%/33.3%, respectively). In conclusion, the treatment of sorted sperm with heparin-PHE in modified drop co-cultures can be recommended for production of in vitro sexed embryos. The use of unsorted sperm for production of embryos of the preferred sex by selected capacitation treatment and co-culture can be the method of choice in bulls with low IVF yields from sorted sperm.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40201 - Animal and dairy science; (Animal biotechnology to be 4.4)
Result continuities
Project
<a href="/en/project/GA17-20405S" target="_blank" >GA17-20405S: Adaptation of control mechanisms of chromosome segregation to dramatic changes during transition from oocytes to early embryos.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Reproduction in domestic animals
ISSN
0936-6768
e-ISSN
1439-0531
Volume of the periodical
56
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
864-871
UT code for WoS article
000636613200001
EID of the result in the Scopus database
2-s2.0-85103597494