Bull sperm capacitation treatment and oocyte-sperm co-culture sysetm affect in vitro bovine embryo development

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000203" target="_blank" >RIV/00027162:_____/20:N0000203 - isvavai.cz</a>

Result on the web

<a href="https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Bull sperm capacitation treatment and oocyte-sperm co-culture sysetm affect in vitro bovine embryo development

Original language description

The study was designed to define the effects of bull sperm treatment and co-culture system on in vitro embryo development. The mature oocytes were fertilized by sperm treated with heparin in standard co-culture (Exp.1) or by sperm treated with heparin supplemented either with PHE mix (penicillamine, hypotaurine, and epinephrine) or caffeine both in standard co-culture and in drops (Exp.2). A variability in the blastocyst rate was observed among bulls after fertilization of oocytes with heparin treated-sperm in standard co-culture. The significantly higher percentages of cleaved oocytes and developed blastocysts were derived from sperm treated with heparin-PHE in comparison with those treated with heparin-caffeine in drops (84.6 and 43.3 vs. 67.6 and 24.8, respectively). The significantly higher percentages of cleaved oocytes and developed blastocysts were recovered from sperm treated with heparin-PHE in drops than from those treated with heparin-caffeine in standard co-culture (85.9 and 47.1 vs. 73.3 and 25.6, respectively). We concluded, that the efficiency of in vitro bovine embryo development depends on sperm capacitation treatment and oocyte-sperm co-culture system.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of 27th International PhD Students Conference

ISBN

978-80-7509-765-1

ISSN

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e-ISSN

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Number of pages

5

Pages from-to

430-434

Publisher name

Mendel University in Brno

Place of publication

Brno

Event location

Brno

Event date

Nov 11, 2020

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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