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Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000110" target="_blank" >RIV/00027162:_____/21:N0000110 - isvavai.cz</a>

  • Alternative codes found

    RIV/60077344:_____/21:00552209 RIV/60460709:41340/21:85343

  • Result on the web

    <a href="https://www.frontiersin.org/articles/10.3389/fvets.2021.659786/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fvets.2021.659786/full</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3389/fvets.2021.659786" target="_blank" >10.3389/fvets.2021.659786</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks

  • Original language description

    Spiroplasma are vertically-transmitted endosymbionts of ticks and other arthropods. Field-collected Ixodes persulcatus have been reported to harbour Spiroplasma, but nothing is known about their persistence during laboratory colonisation of this tick species. We successfully isolated Spiroplasma from internal organs of 6/10 unfed adult ticks, belonging to the third generation of an I. persulcatus laboratory colony, into tick cell culture. We screened a further 51 adult male and female ticks from the same colony for presence of Spiroplasma by genus-specific PCR amplification of fragments of the 16S rRNA and rpoB genes; 100% of these ticks were infected and the 16S rRNA sequence showed 99.8% similarity to that of a previously-published Spiroplasma isolated from field-collected I. persulcatus. Our study shows that Spiroplasma endosymbionts persist at high prevalence in colonised I. persulcatus through at least three generations, and confirms the usefulness of tick cell lines for isolation and cultivation of this bacterium.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    FRONTIERS IN VETERINARY SCIENCE

  • ISSN

    2297-1769

  • e-ISSN

    2297-1769

  • Volume of the periodical

    8

  • Issue of the periodical within the volume

    MAR 26 2021

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    10

  • Pages from-to

    "659786"

  • UT code for WoS article

    000637961700001

  • EID of the result in the Scopus database

    2-s2.0-85103878204