Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000110" target="_blank" >RIV/00027162:_____/21:N0000110 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/21:00552209 RIV/60460709:41340/21:85343
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fvets.2021.659786/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fvets.2021.659786/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fvets.2021.659786" target="_blank" >10.3389/fvets.2021.659786</a>
Alternative languages
Result language
angličtina
Original language name
Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks
Original language description
Spiroplasma are vertically-transmitted endosymbionts of ticks and other arthropods. Field-collected Ixodes persulcatus have been reported to harbour Spiroplasma, but nothing is known about their persistence during laboratory colonisation of this tick species. We successfully isolated Spiroplasma from internal organs of 6/10 unfed adult ticks, belonging to the third generation of an I. persulcatus laboratory colony, into tick cell culture. We screened a further 51 adult male and female ticks from the same colony for presence of Spiroplasma by genus-specific PCR amplification of fragments of the 16S rRNA and rpoB genes; 100% of these ticks were infected and the 16S rRNA sequence showed 99.8% similarity to that of a previously-published Spiroplasma isolated from field-collected I. persulcatus. Our study shows that Spiroplasma endosymbionts persist at high prevalence in colonised I. persulcatus through at least three generations, and confirms the usefulness of tick cell lines for isolation and cultivation of this bacterium.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FRONTIERS IN VETERINARY SCIENCE
ISSN
2297-1769
e-ISSN
2297-1769
Volume of the periodical
8
Issue of the periodical within the volume
MAR 26 2021
Country of publishing house
CH - SWITZERLAND
Number of pages
10
Pages from-to
"659786"
UT code for WoS article
000637961700001
EID of the result in the Scopus database
2-s2.0-85103878204