Spectrum of sperm mtDNA deletions in men exposed to industrial air pollution
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000081" target="_blank" >RIV/00027162:_____/22:N0000081 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1383571822001000" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1383571822001000</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.mrgentox.2022.503538" target="_blank" >10.1016/j.mrgentox.2022.503538</a>
Alternative languages
Result language
angličtina
Original language name
Spectrum of sperm mtDNA deletions in men exposed to industrial air pollution
Original language description
Sperm mtDNA status can serve as a molecular marker of oxidative stress and environmental exposure. High levels of air pollution may be associated with increased mitochondrial DNA (mtDNA) deletion rates in sperm. We compared the length spectra of sperm mtDNA deletions in semen samples collected from city policemen exposed to traffic and industrial air pollution in two seasons with different levels of air pollution. We used long-range PCR to amplify a fragment of mtDNA (8066 bp) frequently affected by deletions, visualized the PCR products by gel electrophoresis, and analysed aberrant bands corresponding to deleted mtDNA, using gel documentation software. The predominance of undeleted sperm mtDNA was accompanied by a variety of shorter PCR product lengths in the vast majority of sperm samples, in both seasons. Sperm mtDNA molecules and bands corresponding to long deletions were more frequently detected than shorter deletions, in both seasons. We did not detect any difference in the total number of electrophoretic bands corresponding to deleted sperm mtDNA and in the number of deleted sperm mtDNA molecules between the two seasons. In our study, air pollution during sperm maturation did not induce formation of large mtDNA deletions detectable by long PCR and gel electrophoresis (>1 kb) in maturing sperm mtDNA.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
<a href="/en/project/EF16_019%2F0000798" target="_blank" >EF16_019/0000798: Healthy Aging in Industrial Environment</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Mutation Research - Genetic Toxicology and Environmental Mutagenesis
ISSN
1383-5718
e-ISSN
1879-3592
Volume of the periodical
882
Issue of the periodical within the volume
October 2022
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
—
UT code for WoS article
000844077700002
EID of the result in the Scopus database
2-s2.0-85135964730